PMID: 8603776Apr 1, 1996Paper

Neutralization of TGF-beta by anti-TGF-beta antibody attenuates kidney hypertrophy and the enhanced extracellular matrix gene expression in STZ-induced diabetic mice

Diabetes
K SharmaF N Ziyadeh

Abstract

Diabetic nephropathy is characterized by renal hypertrophy, thickening of basement membranes, and accumulation of extracellular matrix in the glomerular mesangium and the interstitium. Our previous investigations have shown that high glucose concentration increases transforming growth factor (TGF)-beta1 mRNA in mesangial and proximal tubule cells and that treatment with anti-TGF-beta antibody results in prevention of the effects of high glucose on cell growth (e.g., induction of cellular hypertrophy) and the stimulation of collagen biosynthesis. We evaluated in vivo the functional role of the renal TGF-beta system in diabetic kidney disease by treatment of streptozotocin-induced diabetic mice with either a neutralizing monoclonal antibody against TGF-beta1, -beta2, and -beta3 (alphaT) or nonimmune murine IgG for 9 days. Diabetic mice given IgG demonstrated total kidney and glomerular hypertrophy, significantly elevated urinary TGF-beta1 protein, and increased mRNAs encoding TGF-beta1, type II TGF-beta receptor, alpha1(IV) collagen, and fibronectin. Treatment of diabetic mice with alphaT prevented glomerular hypertrophy, reduced the increment in kidney weight by approximately 50%, and significantly attenuated the increase in mRN...Continue Reading

Citations

Nov 28, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Peter Y ChuangJohn C He
Aug 17, 2011·Cell and Tissue Research·Rosemarie M CarewPhillip Kantharidis
Mar 22, 2012·Journal of Natural Medicines·Qian ZhangHongding Xiang
Nov 13, 2004·Current Diabetes Reports·Tracy A McGowanKumar Sharma
May 31, 2012·Current Diabetes Reports·Anil Karihaloo
Mar 16, 2004·Seminars in Nephrology·Helmy M Siragy
Dec 10, 1999·Diabetes Research and Clinical Practice·S E Bursell, G L King
Jun 26, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G CalvarusoG Tesoriere
Aug 26, 1999·Trends in Endocrinology and Metabolism : TEM·A FlyvbjergA Logan
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S FillonF Lang
Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·I A DarbyD G MacLellan
Mar 10, 2000·Cytokine & Growth Factor Reviews·K Sharma, T A McGowan
Apr 16, 2002·Current Opinion in Pharmacology·Ling YuWayne A Border
Apr 12, 2003·Current Opinion in Pharmacology·Nicholas J Laping
Sep 21, 2001·The Biochemical Journal·N A WahabR M Mason
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SuthanthiranP August
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·W B Reeves, T E Andreoli
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·F LangS Broër
Dec 6, 2006·Journal of Asian Natural Products Research·C-Y HeZ-B Lin
Mar 14, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E E TredgetA Ghahary
Jan 4, 2012·Clinical and Experimental Pharmacology & Physiology·Hui Yao Lan
Apr 8, 2011·The Journal of Pharmacology and Experimental Therapeutics·Mohamed A SalehDavid M Pollock

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.