Neutralizing monoclonal antibodies to an extracellular Pseudomonas cepacia protease.

Infection and Immunity
C KooiP A Sokol

Abstract

Pseudomonas cepacia produces at least two extracellular proteases with apparent molecular masses of 36,000 and 40,000 Da. The 36-kDa protease has high proteolytic activity and the 40-kDa protease has low proteolytic activity with hide powder azure as a substrate. Monoclonal antibodies (MAbs) were raised against the purified 36- and 40-kDa proteases. Several MAbs directed against the 36-kDa protease were found to recognize the 40-kDa protease by Western immunoblot analysis. Similarly, a MAb directed against the 40-kDa protease recognized the 36-kDa protease, suggesting that these two proteases may be immunologically related. A MAb directed against the 36-kDa protease, designated 36-6-8, and a MAb directed against the 40-kDa protease (MAb G-11) cross-reacted with other extracellular proteases, such as Pseudomonas aeruginosa elastase and alkaline protease, Pseudomonas pseudomallei protease, and the Vibrio cholerae hemagglutinin/protease. MAb 36-6-8 neutralized the P. cepacia 36-kDa protease, P. aeruginosa elastase, P. pseudomallei protease, and V. cholerae hemagglutinin/protease but did not affect P. aeruginosa alkaline protease activity. In contrast, MAb G-11 to the 40-kDa protease neutralized only the P. cepacia 36-kDa protease....Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1976·European Journal of Immunology·G KöhlerC Milstein
Oct 1, 1990·Journal of Medical Microbiology·A R Gessner, J E Mortensen
Dec 1, 1989·Molecular Microbiology·C Wandersman
Oct 1, 1987·Molecular & General Genetics : MGG·P HeinrichF Götz
Sep 1, 1986·Infection and Immunity·P A Sokol, D E Woods
Jul 1, 1986·The Journal of Infectious Diseases·B A Booth, R A Finkelstein
Feb 1, 1987·Journal of Clinical Microbiology·T NakazawaM Ishibashi
Jan 21, 1985·Journal of Immunological Methods·L H StankerH Juarez-Salinas
Aug 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·H RinderknechtB J Haverback
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A FinkelsteinP Holt
Sep 1, 1983·Reviews of Infectious Diseases·D E Woods, B H Iglewski
Nov 1, 1983·Infection and Immunity·B A BoothR A Finkelstein
Nov 1, 1983·Reviews of Infectious Diseases·B Wretlind, O R Pavlovskis
Feb 1, 1984·The Journal of Pediatrics·A IslesH Levison
Feb 1, 1984·Journal of Clinical Microbiology·A I McKevitt, D E Woods

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Citations

May 1, 2001·Clinical and Diagnostic Laboratory Immunology·J W SmalleyC A Hart
Dec 10, 2003·Canadian Journal of Microbiology·Nick D AllanTerry J Beveridge
May 12, 2006·Journal of Medical Microbiology·Paul W WhitbyTerrence L Stull
Mar 27, 2004·American Journal of Respiratory and Critical Care Medicine·Anthony De SoyzaRaquel Demarco de Hormaeche
Aug 9, 2003·Microbiology·C R CorbettP A Sokol
Jun 22, 2005·Journal of Bacteriology·C KooiP A Sokol
Jan 28, 1999·Journal of Bacteriology·S LewenzaP A Sokol
Oct 1, 1996·Infection and Immunity·J L BurnsA Griffith

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