Neutrophil adhesive contact dependence on impingement force

Biophysical Journal
C SpillmannRichard E Waugh

Abstract

Neutrophil capture and recruitment from the circulation requires the formation of specific receptor/ligand bonds under hydrodynamic forces. In the present study we examine bond formation between beta2-integrins on neutrophils and immobilized ICAM-1 while using micropipettes to control the force of contact between the cell and substrate. Magnesium was used to induce the high affinity conformation of the integrins, and bond formation was assessed by measuring the probability of adhesion during repeated contacts. Increasing the impingement force caused an increase in the contact area and led to a proportional increase in adhesion probability (from approximately 20 to 50%) over the range of forces tested (50-350 pN). In addition, different-sized beads were used to change the force per unit area in the contact zone (contact stress). We conclude that for a given contact stress, the rate of bond formation increases linearly with contact area, but that increasing contact stress results in higher intrinsic rates of bond formation.

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Citations

Oct 10, 2006·Annals of Biomedical Engineering·Elena B Lomakina, Richard E Waugh
Nov 16, 2011·Journal of Visualized Experiments : JoVE·Veronika I Zarnitsyna, Cheng Zhu
Sep 25, 2007·Biophysical Journal·Christopher D PaschallMichael B Lawrence
Apr 26, 2015·Biomechanics and Modeling in Mechanobiology·Babak GholamiMarco Ellero
Dec 16, 2010·Biophysical Journal·Suman BoseRohit Karnik
Apr 9, 2008·Biophysical Journal·Aleksandr RabodzeyBenoit Ladoux
Aug 8, 2006·Biophysical Journal·Pere Roca-CusachsDaniel Navajas
Jul 8, 2009·Biophysical Journal·Sandrine A HocdéRichard E Waugh
Sep 14, 2004·Biophysical Journal·E B LomakinaR E Waugh
Jan 9, 2018·Technology and Health Care : Official Journal of the European Society for Engineering and Medicine·Atsushi ShiraiJean-Paul Rieu
Sep 22, 2017·Molecular Biology of the Cell·Anna SawickaJulien Husson
Jun 8, 2010·Cellular and Molecular Bioengineering·Jeremy H Snook, William H Guilford
Mar 7, 2020·Biophysical Journal·Luis F DelgadilloRichard E Waugh

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