PMID: 7009756Jan 1, 1981Paper

Neutrophil chemotaxis: technical problems with nitrocellulose filters in Boyden-type chambers

Journal of Immunological Methods
A F Nind

Abstract

The chemotactic migration of murine polymorphonuclear neutrophils (PMN) in Boyden-type chambers was compared in Sartorius membranes which were untreated, or had been autoclaved, and which had been mounted in the chambers in alternative orientations. Migration was assessed by the leading front method. The PMN migrated significantly further in the untreated filters than in filters which had been autoclaved. The orientation of untreated filters in the chamber was critical: PMN migrated less far in filters which had been mounted in their original orientation in the shipping box than in filters which had been inverted before mounting.

References

Jan 1, 1978·Journal of Immunological Methods·T W Jungi

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Citations

Mar 1, 1992·The Journal of protozoology·C Brinkley, F Marciano-Cabral
May 11, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J C ValdézG Perdigón
Feb 1, 1986·The Journal of protozoology·M ClineF Marciano-Cabral
Jul 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Y LeeK Zygourakis

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