PMID: 8944726Nov 1, 1996Paper

Neutrophil depletion attenuates endotoxin-induced dysfunction of cGMP-mediated pulmonary vasorelaxation

The American Journal of Physiology
B SheridanD A Fullerton

Abstract

The effect of neutrophil depletion on endotoxin-induced dysfunction of guanosine 3',5'-cyclic monophosphate (cGMP)-mediated pulmonary vasorelaxation was studied in rats. Two mechanisms of neutrophil depletion were used: vinblastine (0.75 mg/kg iv) and rabbit anti-rat neutrophil antiserum (0.15 ml iv). Concentration-response curves were generated (10(-9) to 10(-6) M) for acetylcholine (ACh), A-23187, and sodium nitroprusside (SNP) in isolated pulmonary arterial rings preconstricted with phenylephrine 6 h after endotoxin (20 mg/kg ip). Absolute neutrophil count was significantly lowered from 1,050 +/- 206 (neutrophils/ml; mean +/- SE) in controls to 100 +/- 41 by vinblastine and to 50 +/- 29 by antiserum. Endotoxin produced histological evidence of pulmonary vascular endothelial damage and significantly increased lung neutrophil accumulation (myeloperoxidase assay, 5.1 +/- 0 vs. 1.2 +/- 0.1 in controls; 0.1 +/- 0.1 and 0.8 +/- 0.0 U/g lung wt after endotoxin in neutrophil-depleted rats by vinblastine and antiserum, respectively). Endotoxin produced significant impairment of endothelium-dependent cGMP-mediated pulmonary vasorelaxation by receptor-dependent (ACh) and -independent (A-23187) pathways as well as endothelium-independen...Continue Reading

Citations

May 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·E J PulidoR C McIntyre
Feb 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M CalkinsR C McIntyre
May 25, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ben M TsaiDaniel R Meldrum
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y SongX Meng
Jan 3, 2001·Journal of Applied Physiology·L C ClavijoR N Garrison
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher D RaeburnXianzhong Meng
Apr 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katherine A BarsnessRobert C McIntyre

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