Neutrophil depletion blocks early collagen degradation in repairing cholestatic rat livers.

The American Journal of Pathology
Mark W HartyThomas F Tracy

Abstract

Biliary obstruction results in a well-characterized cholestatic inflammatory and fibrogenic process; however, the mechanisms and potential for liver repair remain unclear. We previously demonstrated that Kupffer cell depletion reduces polymorphonuclear cell (neutrophil) (PMN) and matrix metalloproteinase (MMP)8 levels in repairing liver. We therefore hypothesized that PMN-dependent MMP activity is essential for successful repair. Male Sprague-Dawley rats received reversible biliary obstruction for 7 days, and the rat PMN-specific antibody RP3 was administered 2 days before biliary decompression (repair) and continued daily until necropsy, when liver underwent morphometric analysis, immunohistochemistry, quantitative RT-PCR, and in situ zymography. We found that RP3 treatment did not reduce Kupffer cell or monocyte number but significantly reduced PMN number at the time of decompression and 2 days after repair. RP3 treatment also blocked resorption of type I collagen. In addition, biliary obstruction resulted in increased expression of MMP3, MMP8, and tissue inhibitor of metalloproteinase 1. Two days after biliary decompression, both MMP3 and tissue inhibitor of metalloproteinase 1 expression declined toward sham levels, whereas...Continue Reading

References

Jan 1, 1991·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·T GaillardK Decker
Sep 11, 1990·European Journal of Biochemistry·K Decker
Dec 1, 1994·The Journal of Surgical Research·P R AldanaT F Tracy
Jul 21, 1998·Journal of Pediatric Gastroenterology and Nutrition·R C Benyon, M J Arthur
Mar 15, 2000·Gut·R C Benyon, J P Iredale
May 4, 2000·The Journal of Surgical Research·K K RogginT F Tracy
Jan 29, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hartmut JaeschkeJohn J Lemasters
Feb 20, 2003·Journal of Hepatology·Scott L Friedman
May 22, 2003·The American Journal of Pathology·Benjamin SchaeferMarcos Rojkind
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jaspreet S GujralHartmut Jaeschke
May 1, 1960·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W C MOLONEYL FLIEGELMAN
Sep 6, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shigeki TsukadaRichard A Rippe

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Citations

Jun 5, 2013·Fibrogenesis & Tissue Repair·Yonggang MaMerry L Lindsey
Mar 21, 2012·Critical Care : the Official Journal of the Critical Care Forum·Adrián González-López, Guillermo M Albaiceta
Apr 17, 2016·The American Journal of Pathology·Charles A Parkos
Apr 26, 2015·Seminars in Immunology·Sarah E Headland, Lucy V Norling
May 9, 2015·International Journal of Inflammation·Florent DuvalDelia Elva Cruz-Vega
May 21, 2016·Frontiers in Immunology·Michelle A SugimotoMauro M Teixeira
Jul 28, 2016·The American Journal of Pathology·Keisaku SatoGianfranco Alpini
Aug 20, 2016·Archives of Toxicology·Tomislav HorvatMaurice Whelan
May 2, 2012·Journal of Leukocyte Biology·Thomas F Tracy
May 1, 2018·American Journal of Respiratory Cell and Molecular Biology·Jorge Blázquez-PrietoGuillermo M Albaiceta
Feb 26, 2014·Nature Reviews. Immunology·Antonella PellicoroJonathan A Fallowfield
Jul 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adrián González-LópezGuillermo M Albaiceta
Nov 15, 2019·Biochemical Society Transactions·Mavis A A TenkorangMerry L Lindsey
Jun 9, 2018·Hepatology Communications·Eiko SaijouAtsushi Miyajima
Jun 18, 2019·The Journal of Clinical Investigation·Moritz Peiseler, Paul Kubes
Nov 4, 2017·Scientific Reports·Maria Francesca SecchiMaurizio Onisto
Nov 8, 2020·Cellular & Molecular Immunology·Kai LiuRuonan Xu
Jul 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Michitaka Matsuda, Ekihiro Seki
Mar 26, 2021·Frontiers in Immunology·Jiaojiao TangHongyang Wang
Jun 26, 2014·Development·Timothy A PetrieRandall T Moon
Jul 13, 2021·Signal Transduction and Targeted Therapy·Huakan ZhaoYongsheng Li
Jun 5, 2017·Advanced Drug Delivery Reviews·Stefania CannitoMaurizio Parola

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