Neutrophil depletion in rats reduces burn-injury induced intestinal bacterial translocation

Critical Care Medicine
Nadeem FazalMohammed M Sayeed

Abstract

To determine whether neutrophil depletion could eradicate intestinal bacterial translocation in bum-injured rats. Prospective, randomized, controlled study. University research laboratory. Adult male Sprague-Dawley rats. The rats were intravenously administered a rabbit anti-rat neutrophil antibody causing profound neutropenia and subjected to a 30% total body surface area scald burn. The depletion of neutrophils from the intestine was assessed via measurements of myeloperoxidase (MPO) activity in the intestinal homogenates. In addition, the presence of activated/extravasated neutrophils in intact intestines was determined via immunohistochemical localization of neutrophil nicotinamide adenine dinucleotide phosphate (NADPH) oxidase component protein p47phox. Bacterial translocation was measured using agar cultures and by determining Escherichia coli beta-galactosidase gene via polymerase chain reaction/Southern blot analyses of mesenteric lymph node and spleen, liver, lung, and blood. MPO measurements demonstrated a six-fold increase above the control value in the intestinal tissue in rats on day 1 postburn. The presence of activated neutrophils (expression of p47phox protein) was also markedly increased in the intestines of th...Continue Reading

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