Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society
J AltrichterT Lögters

Abstract

The predictive value of circulating free DNA/neutrophil extracellular traps (cf-DNA/NETs) has recently been shown in patients with major trauma for sepsis, multiple organ failure, and mortality. Here we report on the predictive potential of cf-DNA/NETs for mortality in patients with severe burn injury. In a prospective study 32 patients with severe burn injury were included. Blood samples were sequentially obtained on day 1, 3, 5, and 7 after admission. cf-DNA/NETs was directly quantified from plasma by means of rapid fluorescence assay. Time kinetics of cf-DNA/NETs were correlated with clinical data, C-reactive protein (CRP), procalcitonin (PCT), and interleukin (IL)-6. Furthermore sensitivity, specificity, and positive and negative predictive value, as well as receiver operation characteristic (ROC) curves were calculated. Seven patients died within the first month after burn injury. cf-DNA/NETs values from these patients were significantly increased already on day 1 and 3 after admission compared with patients who survived (p < 0.01). In contrast, PCT levels of nonsurvivors were significantly elevated on day 3 and 5 (p < 0.01), while CRP and IL-6 did not show any significant difference between survivors and nonsurvivors. At ...Continue Reading

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Citations

Oct 1, 2011·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·N BrusselaersS Blot
Aug 15, 2015·Mediators of Inflammation·Shigeto HamaguchiKazunori Tomono
Aug 12, 2014·Injury·Jon HazeldineJanet M Lord
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Jan 31, 2018·The Journal of Pathology·Caitlin M StewartDana Wy Tsui
Apr 14, 2016·Biological Research for Nursing·Mayu O Frank
Jan 15, 2020·Pediatric Research·Yusuke YoshidaShigeaki Nonoyama
Sep 15, 2020·The Journal of Experimental Medicine·Flavio Protasio VerasFernando Queiroz Cunha
Oct 29, 2015·Critical Care Medicine·Yoshitaka InoueDaniel Irimia
Sep 26, 2019·Frontiers in Immunology·Esmaeil MortazIan M Adcock
Sep 12, 2018·Cell Death and Differentiation·Tom Vanden Berghe, Eric Hoste
Jan 8, 2020·Journal of Translational Medicine·Pei ChenXianghong Jasmine Zhou
Jul 25, 2021·International Journal of Molecular Sciences·Nicole SteffensenNicole de Buhr

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