Neutrophil-derived reactive oxygen species in SSc

Rheumatology
Theresa C BarnesRobert J Moots

Abstract

Reactive oxygen species (ROS) are implicated in the pathogenesis of SSc. Neutrophils constitute a major source of ROS during inflammation. Here, we examined endogenous and stimulated ex vivo ROS production of SSc neutrophils compared with control neutrophils with and without prior priming with TNF-α. ROS generation was measured using luminol-enhanced chemiluminescence. Neutrophils isolated from SSc patients and healthy controls were unprimed or were primed with TNF-α. ROS production was stimulated in vitro with phorbol 12-myristate 13-acetate (PMA) and formyl-met-leu-phe (fMLP). To examine the effects of serum mediators on ROS generation, control neutrophils were also stimulated with SSc or control serum. Neutrophil stimulation with PMA and fMLP resulted in a greater increase in ROS generation in SSc neutrophils compared with controls. However, unstimulated SSc neutrophils generated lower levels of ROS than controls. SSc neutrophils demonstrated an increased response to fMLP in the absence of in vitro TNF-α priming indicating priming of SSc neutrophils in vivo. SSc serum did not stimulate neutrophil ROS generation in vitro. SSc neutrophils are primed for ROS generation. Neutrophils binding to activated endothelium in SSc, may i...Continue Reading

References

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Citations

Apr 15, 2014·Cardiovascular Research·Sophie Van LinthoutCarsten Tschöpe
Dec 24, 2013·Antioxidants & Redox Signaling·Elena OrtonaMarina Pierdominici
Apr 15, 2015·Vascular Pharmacology·Gianluca BagnatoAntonino Saitta
Jul 28, 2015·Biochimica Et Biophysica Acta·Andrew E EkpenyongJochen Guck
Apr 19, 2015·Arthritis Research & Therapy·Giuseppe A RamirezAngelo A Manfredi
Dec 17, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Minrui LiangHejian Zou
Nov 16, 2018·Clinical and Experimental Immunology·M Brown, S O'Reilly
Aug 8, 2018·Frontiers in Immunology·Paoline LaurentCécile Contin-Bordes
Nov 30, 2018·Frontiers in Immunology·Arjan van CaamPeter van der Kraan
Jul 9, 2020·Frontiers in Immunology·Aslıhan Avanoǧlu GülerMarco Matucci-Cerinic
May 22, 2017·Clinical Reviews in Allergy & Immunology·María MorellConcepción Marañón
Apr 29, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Francesca Wanda RossiAmato de Paulis
Jan 20, 2021·The Journal of Clinical Investigation·Michael EliasFlorian Rieder
Jul 27, 2018·Science Translational Medicine·Norma MaugeriAngelo A Manfredi

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