Neutrophil elastase cleaves laminin-332 (laminin-5) generating peptides that are chemotactic for neutrophils

The Journal of Biological Chemistry
Piotr MydelRobert M Senior

Abstract

Proteolytic processing of laminin-332 by matrix metalloproteinase (MMP)-2 and MMP-14 has been shown to yield fragments that are promigratory for epithelial cells. During acute and chronic inflammation, proteases are elaborated by neutrophils and macrophages that can degrade basement membranes. We investigated the susceptibility of laminin-332 to degradation by the following neutrophil and macrophage proteases: neutrophil elastase (NE), cathepsin G, proteinase-3, and MMPs-2, -8, -9, and -12. Protease-specific differences were seen in the capacity to cleave the individual chains of laminin-332. NE and MMP-12 showed the greatest activity toward the gamma2 chain, generating a fragment similar in size to the gamma2x fragment generated by MMP-2. The digestion pattern of laminin-332 by degranulated neutrophils was nearly identical to that generated with NE alone. Digestion by supernatants of degranulated neutrophils was blocked by an inhibitor of NE, and NE-deficient neutrophils were essentially unable to digest laminin-332, suggesting that NE is the major neutrophil-derived protease that degrades laminin-332. In vivo, laminin gamma2 fragments were found in the bronchoalveolar lavage fluid of wild-type mice treated with lipopolysaccha...Continue Reading

References

Oct 1, 1980·The Journal of Clinical Investigation·R M SeniorR P Mecham
Apr 14, 1999·The Journal of Investigative Dermatology·L Borradori, A Sonnenberg
Dec 23, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M FalkP Ekblom
Jan 5, 2000·The Journal of Clinical Investigation·Z LiuL A Diaz
Feb 9, 2000·The Journal of Cell Biology·N KoshikawaV Quaranta
May 12, 2000·The Journal of Biological Chemistry·S AmanoR E Burgeson
Dec 6, 2000·American Journal of Respiratory Cell and Molecular Biology·R A PierceR M Senior
Oct 3, 2002·The EMBO Journal·Claus-Werner FranzkeLeena Bruckner-Tuderman
Mar 13, 2003·Kidney International·Dale R AbrahamsonPatricia L St John
Apr 10, 2003·Biochemical and Biophysical Research Communications·Emma PiriläPäivi Maisi
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tracy L Adair-KirkRobert M Senior
Oct 21, 2003·American Journal of Respiratory Cell and Molecular Biology·Tim O HircheAbderrazzaq Belaaouaj
Nov 25, 2003·The Journal of Investigative Dermatology·Xianmin MengLeena Pulkkinen
Nov 25, 2003·The American Journal of Pathology·Steven D ShapiroAbderazzaq Belaaouaj
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rebecca E YoungSussan Nourshargh
Mar 30, 2004·The Journal of Biological Chemistry·Claus-Werner FranzkeLeena Bruckner-Tuderman
Nov 5, 2004·The Journal of Biological Chemistry·Naohiko KoshikawaMotoharu Seiki
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tracy L Adair-KirkRobert M Senior
Apr 21, 2005·Journal of Cell Science·Shijun WangSussan Nourshargh
May 4, 2005·Cellular and Molecular Life Sciences : CMLS·T SadowskiR Sedlacek
Jun 28, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Monique AumailleyPeter D Yurchenco
Feb 14, 2006·The Journal of Clinical Investigation·A McGarry HoughtonSteven D Shapiro
Feb 14, 2006·Nature Medicine·Nathaniel M WeathingtonJ Edwin Blalock
Jan 4, 2007·The American Journal of Pathology·Felix ChuaGeoffrey J Laurent

❮ Previous
Next ❯

Citations

Oct 7, 2010·International Journal of Clinical Oncology·Nobuyoshi Hiraoka
Sep 3, 2010·Journal of Mammary Gland Biology and Neoplasia·Ori MallerPepper Schedin
Mar 8, 2013·Nature Reviews. Cancer·A McGarry Houghton
Apr 24, 2010·Nature Reviews. Molecular Cell Biology·Sussan NoursharghMichael Sixt
Sep 12, 2008·The Journal of Biological Chemistry·Manisha TripathiVito Quaranta
May 23, 2012·The Journal of Experimental Medicine·Doris ProebstlSussan Nourshargh
Oct 17, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael P ShakarjianJeffrey D Laskin
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christine-Maria HorejsMolly M Stevens
Feb 13, 2014·Pflügers Archiv : European journal of physiology·Yonggang MaMerry L Lindsey
Jun 24, 2014·Journal of Mammary Gland Biology and Neoplasia·Jaime FornettiPepper Schedin
Jan 24, 2016·The Journal of Investigative Dermatology·Jennifer LandsbergClaire Lugassy
Aug 13, 2011·Veterinary Immunology and Immunopathology·Michelle B Visser, Christopher C Pollitt
Jul 30, 2010·Trends in Molecular Medicine·Tanya N MayadasSanjeev Sethi
Jul 31, 2010·Life Sciences·Claude Jourdan-LesauxMerry L Lindsey
Jul 27, 2010·The Veterinary Journal·Geoffroy de la Rebière de Pouyade, Didier Serteyn
Nov 27, 2009·Veterinary Immunology and Immunopathology·Geoffroy de la Rebière de PouyadeDidier Serteyn
Jun 19, 2009·Trends in Biochemical Sciences·Pearl P Y LieDolores D Mruk
May 29, 2010·The Journal of Dermatology·Teruki DainichiTakashi Hashimoto
Apr 11, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Cláudia S MarquesGabriela M Santos-Gomes
Sep 3, 2011·Journal of Molecular and Cellular Cardiology·Tracy DoddPetra Rocic
May 12, 2015·International Journal of Medical Microbiology : IJMM·Josefine HirschfeldIsabelle Bekeredjian-Ding
Jul 20, 2016·American Journal of Reproductive Immunology : AJRI·Masashi TakamuraYutaka Osuga
Sep 2, 2016·The Journal of Clinical Investigation·Amit Gaggar, Nathaniel Weathington
Nov 26, 2008·Pharmacology & Therapeutics·Uros V DjekicNathaniel M Weathington
May 13, 2014·Experimental Dermatology·Sylvie Ricard-Blum, Romain Salza
Feb 7, 2018·International Journal of Molecular Sciences·Daiane BoffPaul Proost
Feb 15, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sigrun EickAndreas Stavropoulos
Jan 24, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natália TavaresCláudia Brodskyn
May 2, 2017·The Journal of Clinical Investigation·Nikolaos G Frangogiannis
Mar 15, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Morten A KarsdalDetlef Schuppan
Jul 13, 2019·Frontiers in Immunology·Sho HiroyasuDavid J Granville

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2021 Meta ULC. All rights reserved