Neutrophil elastase inhibitor reduces asthmatic responses in allergic sheep

Respiration Physiology
K FujimotoK Sugane

Abstract

To determine the role of neutrophil elastase in asthmatic responses, we studied the effect of ONO-5046, a specific neutrophil elastase inhibitor, on antigen-induced asthmatic responses in allergic sheep. Pulmonary resistance (RL) was measured for 8 h after antigen challenge. Measurements of airway responsiveness to methacholine and bronchoalveolar lavage fluid (BALF) were obtained 8 h after challenge. Antigen challenge caused early and late increases in RL, airway hyperresponsiveness (AHR), and recruitment of neutrophils and eosinophils along with increases in TXB2 and LTB4 in BALF. ONO-5046 treatment significantly reduced both early and late bronchoconstriction, neutrophil recruitment, increases in LTB4 in BALF, and AHR. ONO-5046 post-treatment significantly reduced the increase in RL 8 h after antigen challenge. Another neutrophil elastase inhibitor, FR 134043, significantly reduced both early and late bronchoconstriction. ONO-5046 had little effect on calcium ionophore-induced LTB4 release from isolated neutrophils and whole blood obtained from drug-treated sheep. These findings suggest that neutrophil elastase is involved in antigen-induced bronchoconstriction and AHR mediated by neutrophil accumulation and 5-lipoxygenase p...Continue Reading

References

Apr 1, 1991·The American Review of Respiratory Disease·W M AbrahamA Cortes
Jun 14, 1991·Biochemical and Biophysical Research Communications·K KawabataT Miyamoto
Mar 1, 1989·The American Review of Respiratory Disease·H OkayamaT Takishima
Jan 22, 1981·The New England Journal of Medicine·C T LeeG Weinbaum

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Citations

Dec 6, 1997·European Journal of Pharmacology·Y ShinguhT Ono
May 20, 1998·European Journal of Pharmacology·Y ShinguhT Ono
Aug 19, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·N TomizawaY Morishita
Feb 13, 2010·Annual Review of Physiology·John F AlcornJay K Kolls
Dec 8, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Angelia D LockettSusan J Gunst
Aug 8, 2021·International Journal of Molecular Sciences·Juan Antonio Giménez-BastidaJuan Carlos Espín

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