Neutrophil elastase promotes rapid exocytosis in human airway gland cells by producing cytosolic Ca2+ oscillations

American Journal of Respiratory Cell and Molecular Biology
M MaizièresJ Jacquot

Abstract

The molecular and ionic mechanisms responsible for the regulation of mucus exocytosis in human airway gland cells remain poorly defined. To determine whether dynamic changes of intracellular free Ca2+ concentration [Ca2+]i can promote different exocytotic responses, we monitored dynamic changes in [Ca2+]i and secretory granule (SG) exocytosis in individual human tracheal submucosal serous gland (HTG) cells. These changes were in response to exposure of the cells to three different secretagogues associated with airway inflammation and disease: human neutrophil elastase (HNE), histamine, and ATP. Dynamic changes in [Ca2+]i from single cells were determined with Indo-1/AM using quantitative UV laser microspectrofluorometry. The rate of SG exocytosis was measured in single cells by fluorescence videomicroscopy of SG degranulation and by the ELISA method. Exposure of HTG cells to a low concentration of HNE (1.0 microM) caused a high rate of SG exocytosis (52% decrease in the initial quinacrine fluorescence) during the first 8-min stimulation period compared with that observed following exposure of the cells to 100 microM histamine (10% decrease) or 100 microM ATP (6% decrease). In contrast to a rapid and transient rise in [Ca2+]i in...Continue Reading

References

Dec 1, 1992·The American Review of Respiratory Disease·J V FahyJ A Nadel
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·R D RievesJ H Shelhamer
Jan 1, 1992·The American Journal of Physiology·U KachintornK Dharmsathaphorn
May 1, 1991·British Journal of Pharmacology·K C Kim, B C Lee
Nov 11, 1990·Cell Calcium·J W Putney
Jan 1, 1991·Annals of the New York Academy of Sciences·J A Nadel
Mar 1, 1990·The Journal of Clinical Investigation·C P SommerhoffG H Caughey
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Feb 1, 1990·The American Review of Respiratory Disease·Y Sibille, H Y Reynolds
Apr 1, 1988·The Biochemical Journal·M A SelakJ B Smith
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J Breckenridge, W Almers
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E J Campbell
Jan 27, 1995·Cell·D E Clapham
Sep 1, 1993·American Journal of Respiratory Cell and Molecular Biology·M I LethemC W Davis
Apr 1, 1993·American Journal of Respiratory Cell and Molecular Biology·J M Sallenave, A Silva
Dec 1, 1995·American Journal of Respiratory and Critical Care Medicine·T L BonfieldM Berger
Dec 1, 1995·Cell·M D Bootman, M J Berridge

❮ Previous
Next ❯

Citations

Aug 27, 2016·Annals of the American Thoracic Society·Cormac McCarthyNoel G McElvaney
Jun 11, 1999·The American Journal of Physiology·S KanohA Nagai
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·O TabaryJ Jacquot
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·T M Dwyer, J M Farley
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H SaitohK Shirato
Oct 13, 2000·American Journal of Physiology. Cell Physiology·E J ThomasM I Lethem
Jul 17, 1999·The American Journal of Physiology·J H Evans, M J Sanderson
Mar 4, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Derek B McMahonRobert J Lee
May 26, 1998·Biochemical and Biophysical Research Communications·S SebilleM Manfait
May 24, 2000·Cellular Signalling·S Shimura

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.