Neutrophil extracellular traps are associated with disease severity and microbiota diversity in patients with chronic obstructive pulmonary disease

The Journal of Allergy and Clinical Immunology
Alison J DickerJames D Chalmers

Abstract

Neutrophil extracellular traps (NETs) have been observed in the airway in patients with chronic obstructive pulmonary disease (COPD), but their clinical and pathophysiologic implications have not been defined. We sought to determine whether NETs are associated with disease severity in patients with COPD and how they are associated with microbiota composition and airway neutrophil function. NET protein complexes (DNA-elastase and histone-elastase complexes), cell-free DNA, and neutrophil biomarkers were quantified in soluble sputum and serum from patients with COPD during periods of disease stability and during exacerbations and compared with clinical measures of disease severity and the sputum microbiome. Peripheral blood and airway neutrophil function were evaluated by means of flow cytometry ex vivo and experimentally after stimulation of NET formation. Sputum NET complexes were associated with the severity of COPD evaluated by using the composite Global Initiative for Obstructive Lung Disease scale (P < .0001). This relationship was due to modest correlations between NET complexes and FEV1, symptoms evaluated by using the COPD assessment test, and higher levels of NET complexes in patients with frequent exacerbations (P = .0...Continue Reading

References

Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·C Haslett
Dec 4, 2002·Nature Reviews. Immunology·John SavillChris Haslett
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Nov 3, 2005·The European Respiratory Journal·B W M WillemseW Timens
Feb 23, 2007·The New England Journal of Medicine·Peter M A CalverleyUNKNOWN TORCH investigators
Jan 19, 2008·The American Journal of Pathology·Sarah L FarnworthTariq Sethi
Mar 7, 2009·American Journal of Respiratory and Critical Care Medicine·Tiffany R RichensR William Vandivier
May 19, 2009·Nature Medicine·Kai KessenbrockDieter E Jenne
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Sep 4, 2010·American Journal of Respiratory and Critical Care Medicine·Nancy K LeidyUNKNOWN EXACT-PRO Study Group
Oct 27, 2010·The Journal of Cell Biology·Venizelos PapayannopoulosArturo Zychlinsky
Feb 15, 2011·The Journal of Allergy and Clinical Immunology·Ryszard DworskiShida Yousefi
Jun 18, 2011·American Journal of Respiratory and Critical Care Medicine·Mona BafadhelChristopher E Brightling
Jan 3, 2012·Blood·Renzo MenegazziPietro Dri
Apr 5, 2012·Chest·Louise E Donnelly, Peter J Barnes
Jun 12, 2012·The Journal of Clinical Investigation·Axelle CaudrillierMark R Looney
Aug 11, 2012·American Journal of Respiratory and Critical Care Medicine·Jørgen VestboRoberto Rodriguez-Roisin
Jan 19, 2013·American Journal of Respiratory Cell and Molecular Biology·Kim Hoenderdos, Alison Condliffe
Mar 16, 2013·American Journal of Respiratory and Critical Care Medicine·Alison MorrisUNKNOWN Lung HIV Microbiome Project
Mar 29, 2013·Science Translational Medicine·Ritika KhandpurMariana J Kaplan
May 7, 2013·The European Respiratory Journal·Alvar AgustiRobert Rodriguez-Roisin
Jun 6, 2013·Expert Review of Respiratory Medicine·Robert P DicksonGary B Huffnagle
Sep 7, 2013·Blood·Bryan G Yipp, Paul Kubes
Apr 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaoning JiangJaroslaw W Zmijewski
Dec 9, 2014·American Journal of Respiratory and Critical Care Medicine·David M SayahMark R Looney
Apr 2, 2015·American Journal of Respiratory and Critical Care Medicine·Bartolome R CelliUNKNOWN ATS/ERS Task Force for COPD Research
Jul 15, 2015·The New England Journal of Medicine·Peter LangeJørgen Vestbo
Jul 15, 2015·The New England Journal of Medicine·Frank E Speizer, James H Ware

❮ Previous
Next ❯

Citations

Oct 4, 2018·Respirology : Official Journal of the Asian Pacific Society of Respirology·Steven L TaylorGeraint B Rogers
Dec 6, 2018·Advanced Healthcare Materials·Midori MaedaShuichi Takayama
Apr 28, 2018·The European Respiratory Journal·Robert D Gray
Mar 2, 2018·European Respiratory Review : an Official Journal of the European Respiratory Society·Shruthi RavimohanGregory P Bisson
Sep 29, 2018·American Journal of Respiratory and Critical Care Medicine·Micheál Mac AogáinSanjay H Chotirmall
Mar 23, 2019·Blood·Fernanda V S Castanheira, Paul Kubes
Mar 23, 2019·Blood·Dachuan Zhang, Paul S Frenette
Feb 1, 2018·Nature Reviews. Gastroenterology & Hepatology·Masaki Honda, Paul Kubes
Aug 3, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Jodie L Simpson
Oct 24, 2018·International Journal of Molecular Sciences·Leonardo TerranovaFrancesco Blasi
May 10, 2019·F1000Research·Alice E JasperGeorgia M Walton
Apr 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shun-Chin YangTsong-Long Hwang
May 6, 2020·The European Respiratory Journal·James D ChalmersMarc Miravitlles
Sep 12, 2019·Cell Death & Disease·Nicole de BuhrMaren von Köckritz-Blickwede
Sep 15, 2020·The Journal of Experimental Medicine·Flavio Protasio VerasFernando Queiroz Cunha
Feb 26, 2019·Frontiers in Immunology·Mohib UddinFrauke Pedersen
Sep 6, 2019·European Respiratory Review : an Official Journal of the European Respiratory Society·Hollian RichardsonJames D Chalmers
Nov 7, 2019·American Journal of Physiology. Cell Physiology·Shi B ChiaYvonne M W Janssen-Heininger
Dec 6, 2019·Breathe·Diego Severiche-BuenoSanjay H Chotirmall
Feb 8, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C PorsbjergP G Gibson
Feb 21, 2018·Frontiers in Immunology·Tamara HoppenbrouwersJohan W van Neck
Sep 7, 2019·International Journal of Molecular Sciences·Botond SzucsJanos T Varga
Aug 9, 2019·Expert Review of Respiratory Medicine·Michael J HughesRobert Stockley
Aug 18, 2018·Mucosal Immunology·Gopinath KasettyArne Egesten
Jul 3, 2019·American Journal of Respiratory and Critical Care Medicine·Simon FinchJames D Chalmers
May 3, 2019·The Journal of Rheumatology·Mi-Hyun AhnHyoun-Ah Kim
Aug 1, 2019·Respiratory Research·Samantha J ThulbornMona Bafadhel
May 2, 2020·Cell Death & Disease·Shida YousefiHans-Uwe Simon
Dec 7, 2019·The European Respiratory Journal·Denise J WoodingUNKNOWN Canadian Respiratory Research Network
Aug 29, 2020·The European Respiratory Journal·David Connell, Anand Shah
Jul 3, 2020·Respiratory Research·Mike LonerganJames D Chalmers
Dec 19, 2019·Frontiers in Immunology·Vanessa GrangerLuc de Chaisemartin

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
ELISA
coronary artery bypass
flow cytometry
PMA
bronchoalveolar lavage

Software Mentioned

Quantitative Insights in Microbial Ecology

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Respirology : Official Journal of the Asian Pacific Society of Respirology
Thomas K WrightKatherine J Baines
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Cuiping HuangHui Gao
BMJ : British Medical Journal
Christina GeorgeJohn R Hurst
© 2022 Meta ULC. All rights reserved