Neutrophil extracellular traps induce aggregation of washed human platelets independently of extracellular DNA and histones

Cell Communication and Signaling : CCS
Omar ElaskalaniPat Metharom

Abstract

The release of neutrophil extracellular traps (NETs), a mesh of DNA, histones and neutrophil proteases from neutrophils, was first demonstrated as a host defence against pathogens. Recently it became clear that NETs are also released in pathological conditions. NETs released in the blood can activate thrombosis and initiate a cascade of platelet responses. However, it is not well understood if these responses are mediated through direct or indirect interactions. We investigated whether cell-free NETs can induce aggregation of washed human platelets in vitro and the contribution of NET-derived extracellular DNA and histones to platelet activation response. Isolated human neutrophils were stimulated with PMA to produce robust and consistent NETs. Cell-free NETs were isolated and characterised by examining DNA-histone complexes and quantification of neutrophil elastase with ELISA. NETs were incubated with washed human platelets to assess several platelet activation responses. Using pharmacological inhibitors, we explored the role of different NET components, as well as main platelet receptors, and downstream signalling pathways involved in NET-induced platelet aggregation. Cell-free NETs directly induced dose-dependent platelet ag...Continue Reading

References

May 15, 1983·Thrombosis Research·M Radomski, S Moncada
Dec 7, 1983·Biochimica Et Biophysica Acta·E M BeversR F Zwaal
Jul 1, 1983·Thrombosis Research·P K PalM M Gertler
Oct 1, 1981·Thrombosis Research·C A Dorsch
Mar 4, 2000·The Journal of Biological Chemistry·G R SambranoS R Coughlin
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Q LuK J Clemetson
Dec 3, 2005·The Journal of Clinical Investigation·Joel S Bennett
Nov 17, 2006·Annals of the New York Academy of Sciences·Anurag GuptaSinuhe Hahn
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Abdul HakkimArturo Zychlinsky
Aug 3, 2010·Nature Medicine·Steffen MassbergBernd Engelmann
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobias A FuchsDenisa D Wagner
Nov 17, 2010·Pharmacological Reviews·Brice KorkmazFrancis Gauthier
Jun 28, 2011·Blood·Tobias A FuchsDenisa D Wagner
Jun 30, 2011·Journal of Thrombosis and Haemostasis : JTH·C T AmmolloC T Esmon
Aug 16, 2011·Journal of Thrombosis and Haemostasis : JTH·L L SwystunP C Liaw
Nov 3, 2011·Journal of Thrombosis and Haemostasis : JTH·A BrillDenisa D Wagner
Mar 28, 2012·The Journal of Experimental Medicine·Marie-Luise von BrühlSteffen Massberg
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mélanie DemersDenisa D Wagner
Feb 26, 2013·Nature Reviews. Immunology·Elzbieta Kolaczkowska, Paul Kubes
Apr 23, 2013·Free Radical Biology & Medicine·H KolarovaL Kubala
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kimberly MartinodDenisa D Wagner
Aug 24, 2013·Thrombosis and Haemostasis·A CarestiaM Schattner
Oct 15, 2013·Basic & Clinical Pharmacology & Toxicology·Melania DovizioPaola Patrignani
Dec 4, 2013·Thrombosis Research·Eduardo FuentesIván Palomo
Jul 12, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Travis J GouldPatricia C Liaw
Apr 4, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Pat MetharomRobert K Andrews
May 6, 2015·Journal of Visualized Experiments : JoVE·Sara NajmehLorenzo E Ferri
Jun 16, 2015·Nature Medicine·Siu Ling WongDenisa D Wagner
Mar 2, 2017·International Journal of Molecular Sciences·Norbaini Abdol RazakPat Metharom

❮ Previous
Next ❯

Citations

Jan 19, 2019·Cancers·Caroline J ReddelVivien M Chen
Jan 4, 2020·Journal of Thrombosis and Thrombolysis·Richard C BeckerSakthivel Sadayappan
Jul 31, 2020·Experimental Biology and Medicine·Mostafa N Mostafa, Mahmoud Osama
Jul 10, 2019·Frontiers in Cardiovascular Medicine·Amanda Z Zucoloto, Craig N Jenne
Aug 6, 2019·Frontiers in Immunology·Alice AssingerJulie Rayes
Dec 5, 2019·Scientific Reports·Joost C de VriesCoen Maas
Apr 17, 2020·Journal of Thrombosis and Haemostasis : JTH·Julia TilburgGrace M Thomas
May 18, 2020·Journal of Thrombosis and Thrombolysis·Richard C Becker
Oct 1, 2020·Biomolecules·Ines Martin-MartinEric Calvo
Aug 11, 2020·Thrombosis and Haemostasis·Thorsten KesslerPhilipp von Hundelshausen
Feb 26, 2020·International Journal of Molecular Sciences·Alexandre MansourIsabelle Gouin-Thibault
Jan 28, 2020·Platelets·Ricardo M GómezMirta Schattner
Jan 16, 2021·Journal of Thrombosis and Thrombolysis·Iraklis C Moschonas, Alexandros D Tselepis
Feb 12, 2021·Progress in Lipid Research·Ilaria CasariMarco Falasca
Aug 3, 2020·Life Sciences·Gerwyn MorrisMichael Berk
May 27, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Monica A BrayRolando E Rumbaut
Jun 5, 2020·Biomacromolecules·Chanel C LaJayachandran N Kizhakkedathu
Jul 10, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Ann S KimKeith R McCrae
Apr 4, 2021·The Journal of Investigative Dermatology·Franziska HersterAlexander N R Weber
May 1, 2021·International Journal of Molecular Sciences·María Amparo Blanch-RuizÁngeles Álvarez
Mar 21, 2021·Thrombosis and Haemostasis·Jun WanMark Roest
Mar 20, 2021·Tissue Engineering. Part B, Reviews·Allison E Fetz, Gary L Bowlin
May 24, 2021·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali Komi, Wolfgang M Kuebler
Jun 3, 2021·Biomolecules·Brandon Bautista-BecerrilAngel Camarena
Aug 28, 2021·International Journal of Molecular Sciences·Monika Szturmowicz, Urszula Demkow
Dec 24, 2021·Hämostaseologie·Nadine GauchelDaniel Duerschmied

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PMA
flow cytometry
electrophoresis
light

Software Mentioned

Chrono
log

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.