Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing

Parasite Immunology
R N Incani, D J McLaren

Abstract

The capacity of rat peritoneal neutrophils to adhere to and kill schistosomula of Schistosoma mansoni in vitro has been investigated. Neutrophils adhere readily to schistosomula in the presence of antibody plus complement (C) and C alone (fresh normal rat serum), but not with heat-inactivated normal rat serum. However, schitosomular killing is only achieved with neutrophils and fIRS or fNRS. In the presence of hiIRS the cells detach after 6 h without producing a significant level of parasite death. The system involving neutrophils plus fIRS is the most efficient in terms of serum dilution and the rate of schistosomular killing. The complement-dependent antibody involved in this system belongs to the class IgG and occurs in rat serum at peak titres, 6-8 wk after a primary schistosome infection. Neutrophil adherence in the presence of fNRS depends upon the generation of C3b molecules at the parasite surface via the alternative pathway of C activation. Studies on the antibody alone system indicate that the lack of significant schistosomular killing might result from the absence of factors which stimulate cell migration, since if a chemokinetic agent is introduced into the assay a 30% increase in mortality is recorded. The possible...Continue Reading

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Related Concepts

Metazoa
Antibody-Dependent Cellular Cytotoxicity
Hemolytic Complement
Immunologic Tumoricidal Activities
Immune Adherence Reaction
Neutrophil Band Cells
Schistosoma mansoni
Mouse, Swiss
In Vitro [Publication Type]

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