PMID: 7514099Apr 1, 1994Paper

Neutrophil mediated damage to isolated myocytes after anoxia and reoxygenation

Cardiovascular Research
P R Hansen, G Stawski

Abstract

The aim was to assess the role of neutrophils in anoxia-reoxygenation induced, neutrophil mediated damage to cardiac myocytes. Neonatal rat cardiac myocytes in primary monolayer cultures were exposed to a 2 h period of anoxia, and subsequently reoxygenated for 3 h. Neutrophils were added at the time of reoxygenation, and myocyte injury was determined by release of lactate dehydrogenase (LDH). Neutrophils produced a dose dependent increase in myocyte LDH release. This effect was not enhanced by coincubation with a neutrophil activator (formyl-Met-Leu-Phe), or interleukin 1 alpha (IL-1 alpha), although IL-1 alpha increased anoxic myocyte damage. Exposure to supernatants from anoxic, or anoxic-reoxygenated, myocytes increased LDH release from normoxic myocytes, and conditioning of these supernatants by neutrophils further increased their cytotoxic potential. The anoxia-reoxygenation induced, neutrophil mediated LDH release was attenuated by some oxygen radical scavengers (superoxide dismutase, histidine, and desferrioxamine), but not others (catalase). Marked decrease in LDH release was also observed after addition of L-arginine, the substrate for synthesis of nitric oxide, along with the neutrophils at the time of reoxygenation. ...Continue Reading

Citations

Mar 15, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A CharmanP R Belcher
Feb 1, 1997·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·V L PathiD J Wheatley
Sep 6, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Mark L DaCostaValluvan Jeevanandam
Jan 30, 2007·Hematology/oncology Clinics of North America·Jakob Vinten-JohansenLynetta J Jobe
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