Abstract
The aim was to assess the role of neutrophils in anoxia-reoxygenation induced, neutrophil mediated damage to cardiac myocytes. Neonatal rat cardiac myocytes in primary monolayer cultures were exposed to a 2 h period of anoxia, and subsequently reoxygenated for 3 h. Neutrophils were added at the time of reoxygenation, and myocyte injury was determined by release of lactate dehydrogenase (LDH). Neutrophils produced a dose dependent increase in myocyte LDH release. This effect was not enhanced by coincubation with a neutrophil activator (formyl-Met-Leu-Phe), or interleukin 1 alpha (IL-1 alpha), although IL-1 alpha increased anoxic myocyte damage. Exposure to supernatants from anoxic, or anoxic-reoxygenated, myocytes increased LDH release from normoxic myocytes, and conditioning of these supernatants by neutrophils further increased their cytotoxic potential. The anoxia-reoxygenation induced, neutrophil mediated LDH release was attenuated by some oxygen radical scavengers (superoxide dismutase, histidine, and desferrioxamine), but not others (catalase). Marked decrease in LDH release was also observed after addition of L-arginine, the substrate for synthesis of nitric oxide, along with the neutrophils at the time of reoxygenation. ...Continue Reading
Citations
Jan 1, 1995·Molecular Aspects of Medicine·P AscenziE Menegatti
Mar 15, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A CharmanP R Belcher
Feb 1, 1997·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·V L PathiD J Wheatley
Sep 6, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Mark L DaCostaValluvan Jeevanandam
Jan 30, 2007·Hematology/oncology Clinics of North America·Jakob Vinten-JohansenLynetta J Jobe
Jul 1, 1996·American Heart Journal·B ZhuC L Wolfe
Feb 3, 2015·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Vivian G NgGregg W Stone
Jul 12, 2001·Japanese Circulation Journal·T AtsuumiY Maruyama
Jan 13, 2005·Canadian Journal of Physiology and Pharmacology·Satoshi Takeo, Kouichi Tanonaka
Dec 26, 2001·Anesthesiology·Gaëlle ClermontClaude Girard
Nov 5, 1997·Plastic and Reconstructive Surgery·P G CordeiroR E Kirschner
Mar 15, 1995·Circulation·P R Hansen
Aug 1, 1996·Circulation·E A GillL D Horwitz
Jun 12, 1999·Circulation Research·B Y PoonP Kubes
May 9, 2006·Journal of Cardiovascular Pharmacology·Farzad EbrahimiAhmad Reza Dehpour
May 11, 2006·Journal of Cardiac Surgery·Michael F SwartzGary F Nieman
Aug 18, 1999·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·L Birk-SørensenC Wang
Jul 17, 1999·The American Journal of Physiology·M G Simms, K R Walley