PMID: 9175919Jun 1, 1997Paper

Neutrophil-mediated damage to vascular endothelium in the spontaneously hypertensive rat

Clinical Immunology and Immunopathology
W Ofosu-AppiahT Richard

Abstract

Lysis of aortic endothelial cells (EC) by neutrophils from spontaneously hypertensive rats (SHR) was investigated using a nonradioactive cytotoxicity assay. Interleukin-1-activated EC, but not unstimulated EC, were effective target cells for lysis by SHR neutrophils. Supernatants from activated neutrophil did not exert a cytotoxic effect on EC. Inhibitors of reactive oxygen species did not affect the cytotoxicity of neutrophils on EC. In contrast, inhibitors of serine protease and elastase markedly inhibited the cytotoxicity of neutrophils on EC. Antibodies against the endothelial cell surface ligands ICAM-1 (CD54) and E-selectin (CD62E) inhibited the adhesion and cytotoxicity of activated neutrophils on EC. The cytotoxicity of neutrophils required direct cell-to-cell contact because separating them with a microporous membrane abrogated the neutrophil-mediated cytotoxic activity. These results demonstrate that SHR neutrophils possess potent cytotoxicity against cytokine-activated EC. Neutrophil-mediated damage of EC could contribute to organ damage in hypertension under conditions of local or systemic activation of neutrophils.

References

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Citations

Jan 6, 2001·The American Journal of Pathology·A M JoussenA P Adamis
Dec 5, 2002·Shock·Masakatsu GotoMohammed M Sayeed
Jul 11, 2007·Journal of Hypertension·Kate M EdwardsPaul J Mills
Apr 6, 2011·Journal of Vascular Research·Sheng TongGeert W Schmid-Schönbein
Oct 11, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Kyo C MunGeert W Schmid-Schönbein
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Sacha ZeerlederWalter A Wuillemin
Sep 17, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Carlos Menendez-CastroKarl F Hilgers

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