Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism

Proceedings of the National Academy of Sciences of the United States of America
Kit WongHenry Bourne

Abstract

Chemoattractants like fMet-Leu-Phe (fMLP) induce neutrophils to polarize with phosphatidylinositol 3,4,5-trisphosphate (PIP3) and protrusive F-actin at the front and actomyosin contraction at the sides and back. RhoA and its downstream effector, myosin II, mediate the "backness" response, which locally inhibits the "frontness" response and constrains its location to one part of the cell. In living HL-60 cells, we used a fluorescent PIP3 probe or a single-chain FRET biosensor for RhoA-GTP to assess spatial distribution of frontness or backness responses, respectively, during the first 3 min after exposure to a uniform concentration of fMLP. Increased PIP3 signal or RhoA activity initially localized randomly about the cell's periphery but progressively redistributed to the front or to the back and sides, respectively. Cells rendered unable to mount the frontness response (by inhibiting actin polymerization or Gi, a trimeric G protein) responded to a micropipette source of attractant by localizing RhoA activity at the up-gradient edge. We infer that protrusive F-actin, induced by the frontness response, constrains the spatial distribution of backness by locally reducing activation of RhoA, thereby reducing its active form at the f...Continue Reading

References

Aug 1, 1979·The Journal of Cell Biology·S H Zigmond, S J Sullivan
Apr 10, 1999·Molecular Biology of the Cell·G ServantH R Bourne
Feb 2, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S S Andersen, G Q Bi
Jun 14, 2000·Immunologic Research·G M Bokoch
Oct 13, 2000·Science·V S KraynovK M Hahn
Oct 26, 2000·Trends in Cell Biology·P RickertG Servant
Sep 12, 2001·Trends in Biochemical Sciences·C Y ChungR A Firtel
Mar 14, 2002·Current Opinion in Cell Biology·Orion D Weiner
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Anna Barbara HauertVerena Niggli
Jun 25, 2002·Nature Cell Biology·Orion D WeinerHenry R Bourne
Jan 29, 2003·The Journal of Cell Biology·Supriya SrinivasanHenry R Bourne
Mar 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·M A LokutaA Huttenlocher
Apr 4, 2003·The Journal of Biological Chemistry·Peter Devreotes, Chris Janetopoulos
Aug 2, 2003·Journal of Cell Science·Sylvain Merlot, Richard A Firtel
Sep 10, 2003·The International Journal of Biochemistry & Cell Biology·Verena Niggli
Oct 2, 2003·Current Opinion in Cell Biology·Masaki FukataKozo Kaibuchi
Dec 31, 2003·Developmental Biology·Myrto Raftopoulou, Alan Hall
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg
Mar 20, 2004·Journal of Neurochemistry·Céline MénagerKozo Kaibuchi
Apr 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jingsong XuHenry R Bourne

❮ Previous
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Citations

Nov 10, 2012·Pflügers Archiv : European journal of physiology·Charlene DepryJin Zhang
Dec 23, 2011·Annals of Biomedical Engineering·Beum Jun Kim, Mingming Wu
Apr 26, 2007·Bulletin of Mathematical Biology·Alexandra JilkineLeah Edelstein-Keshet
Dec 15, 2010·Journal of the American Chemical Society·Nobuhiro UmedaTakanari Inoue
Aug 2, 2006·Nature Cell Biology·Keith Burridge, Renee Doughman
Dec 17, 2009·Nature Cell Biology·Elena KardashErez Raz
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·Rong Li, Gregg G Gundersen
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·David M Bryant, Keith E Mostov
May 21, 2010·Nature Reviews. Molecular Cell Biology·Leif Dehmelt, Philippe I H Bastiaens
Oct 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Dustin KhiemBrian L Black
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mikiko InakiPernille Rørth
Feb 23, 2007·Molecular Biology of the Cell·Frank van den BoomMartin Bähler
Dec 29, 2006·Molecular Biology of the Cell·Paul A NuzziAnna Huttenlocher
Oct 12, 2007·Molecular Biology of the Cell·Mary A LokutaAnna Huttenlocher
Aug 8, 2008·Molecular Biology of the Cell·Teruko NishiokaMichiyuki Matsuda
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Fei Wang
Sep 16, 2009·Blood·Kathleen SzczurMarie-Dominique Filippi
Apr 24, 2010·Journal of Leukocyte Biology·Carla CarboDenisa D Wagner
Mar 21, 2007·PLoS Computational Biology·Matthew Onsum, Christopher V Rao
May 10, 2011·PLoS Computational Biology·Alexandra Jilkine, Leah Edelstein-Keshet
Jan 10, 2013·PLoS Computational Biology·William R Holmes, Leah Edelstein-Keshet
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yu WangMiho Iijima
Mar 8, 2008·FEBS Letters·E A Papakonstanti, C Stournaras
Jul 26, 2006·The Journal of Cell Biology·Alexandra Van KeymeulenHenry R Bourne
Dec 19, 2007·The Journal of Cell Biology·Kit WongHenry R Bourne
Mar 21, 2007·The Journal of Cell Biology·Taeck J JeonRichard A Firtel
Jun 19, 2008·The Journal of Cell Biology·Hsiao-Hui Lee, Zee-Fen Chang
Dec 23, 2015·Nature Cell Biology·Hee Won YangTobias Meyer
Oct 4, 2015·Current Opinion in Cell Biology·Alexander B Verkhovsky
Nov 15, 2011·Current Opinion in Plant Biology·Yoshihisa Oda, Hiroo Fukuda
Jan 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Harrison V Prentice-MottJagesh V Shah
Nov 17, 2009·Current Opinion in Chemical Biology·Lisa M DiPilato, Jin Zhang
Dec 5, 2015·Biophysical Journal·Tenghu Wu, James J Feng
May 6, 2015·Small GTPases·Carlo C CampaEmilio Hirsch
Dec 11, 2007·Journal of Theoretical Biology·Thomas RiggsDenise E Kirschner
Aug 19, 2007·Experimental Cell Research·Ann P Wheeler, Anne J Ridley
Jul 14, 2007·Current Opinion in Genetics & Development·Hans Meinhardt
Jan 24, 2014·Traffic·Dayanidhi RamanAnn Richmond
Oct 30, 2013·Biotechnology Journal·Clotilde Randriamampita, Annemarie C Lellouch
Apr 5, 2014·Developmental Cell·Oliver HoellerOrion D Weiner
Jan 24, 2008·Biophysical Journal·Yoichiro MoriLeah Edelstein-Keshet

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