Neutrophil transendothelial migration potential predicts rejection severity in human cardiac transplantation

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
David G HealyA E Wood

Abstract

Transplant rejection remains a clinical problem despite therapies that focus on lymphocyte suppression, with little attention focused on the neutrophil. Neutrophils are however the first leukocyte to infiltrate the allograft, are capable of causing myocardial damage and may facilitate lymphocytes recruitment. We hypothesised that an early allograft neutrophil infiltration influences rejection severity. Myocardial neutrophil infiltration was assessed using CD15 and myeloperoxidase immunohistochemistry of rejection surveillance endomyocardial biopsy specimens from human cardiac transplant recipients (n=18). In patients undergoing cardiac transplantation (n=10), neutrophils were isolated from multiple perioperative blood samples using a ficoll-based density gradient centrifugation method. The expression of the neutrophil adhesion protein CD11b was then assessed using flow cytometry and compared to subsequent endomyocardial biopsy rejection grades. The effects of contemporary immunosuppressive agents on human neutrophil CD11b were also assessed using healthy control volunteers. Myeloperoxidase staining of endomyocardial biopsies from human heart transplant recipients demonstrated a positive correlation between the degree of neutrop...Continue Reading

Citations

Jul 14, 2010·Transplantation Proceedings·D G HealyA E Wood
Sep 29, 2009·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Alan SooR William G Watson
Nov 26, 2008·Transplantation Reviews·Fleur Samantha BenghiatAlain Le Moine
Feb 6, 2007·Transplantation Proceedings·D G HealyA E Wood
Feb 15, 2013·Immunological Reviews·Zhongmin LiuShuiping Jiang
Mar 28, 2012·European Journal of Clinical Investigation·Susan A KennedyR William G Watson
Feb 23, 2008·Free Radical Biology & Medicine·Galen M Pieper, Allan M Roza
Apr 1, 2017·Journal of Cardiothoracic Surgery·Yie Roei CheeDavid G Healy
Oct 30, 2008·Journal of Immunotoxicology·Russell L Dedrick
Jan 21, 2017·Cell Transplantation·Kevin P KennellyDavid J Keegan
May 31, 2018·The Korean Journal of Internal Medicine·Byung Ha ChungMi-La Cho

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