Neutrophils augment LPS-mediated pro-inflammatory signaling in human lung epithelial cells

Biochimica Et Biophysica Acta
Agnes W BootsRoel P F Schins

Abstract

The role of polymorphonuclear neutrophils in pulmonary host defense is well recognized. The influence of a pre-existing inflammation driven by neutrophils (neutrophilic inflammation) on the airway epithelial response toward pro-inflammatory exogenous triggers, however, is still poorly addressed. Therefore, the aim of the present study is to investigate the effect of neutrophils on lipopolysaccharide (LPS)-induced pro-inflammatory signaling in lung epithelial cells. Additionally, underlying signaling pathways are examined. Human bronchial epithelial cells (BEAS-2B) were co-incubated with human peripheral blood neutrophils or bone-marrow derived neutrophils from either C57BL/6J wild type or nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase deficient (p47(phox-/-)) mice. Upon stimulation with LPS, interleukin (IL)-8 production and reactive oxygen species (ROS) generation were measured. Additionally, activation of the extracellular signal-regulated kinases (ERK) 1/2 and nuclear factor (NF)-κB signaling pathways was analyzed. Our studies show that the presence of neutrophils synergistically increases LPS-induced IL-8 and ROS production by BEAS-2B cells without inducing cytotoxicity. The observed IL-8 response to endotoxin ...Continue Reading

References

Jan 1, 1991·Experimental Lung Research·S L KunkelR M Strieter
Oct 1, 1989·The Journal of Clinical Investigation·M BaggioliniS L Kunkel
Mar 1, 1973·The Journal of Clinical Investigation·B M BabiorJ T Curnutte
Oct 23, 1997·The Journal of Clinical Investigation·A GörlachJ T Curnutte
Aug 12, 1999·Free Radical Biology & Medicine·A M KnaapenP J Borm
Sep 2, 1999·Biochemical and Biophysical Research Communications·H TatsuzawaM Nakano
Jun 6, 2000·American Journal of Respiratory Cell and Molecular Biology·C GersonG E Conner
Jun 30, 2000·The Journal of Biological Chemistry·D L SegevS Maheswaran
Jun 21, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J Thorn
Nov 9, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sekhar P M Reddy, Brooke T Mossman
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Miklós GeisztThomas L Leto
Dec 25, 2003·Journal of Leukocyte Biology·Rachel BoxioOliver Nüsse
Feb 21, 2004·American Journal of Respiratory Cell and Molecular Biology·Daya UpadhyayDavid W Kamp
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Quan ShaRobert P Schleimer
Jun 29, 2004·Treatments in Respiratory Medicine·Suil Kim, Jay A Nadel
Oct 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fiona M MoodieIrfan Rahman
Jan 11, 2005·The European Respiratory Journal·A LindénG P Anderson
Jan 15, 2005·Antioxidants & Redox Signaling·Samuel SigaudBeatriz González-Flecha
Dec 24, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yu-Mee KimJames M Samet
Apr 14, 2006·Current Topics in Medicinal Chemistry·Caroline GuayGuy M Tremblay
Nov 1, 2006·Critical Reviews in Immunology·Yoshiro Kobayashi
Dec 1, 2006·The Journal of Biological Chemistry·Umadevi V WesleyAlbert van der Vliet
Jan 16, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Takashi NakanagaMatt X G Shao
Apr 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nejla GüngörAd M Knaapen
Aug 21, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linda A Tephly, A Brent Carter
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Yoshiro Kobayashi
Dec 7, 2007·The European Respiratory Journal·A HaegensE F M Wouters
Jan 1, 2008·Free Radical Biology & Medicine·Albert van der Vliet
Apr 12, 2008·American Journal of Respiratory Cell and Molecular Biology·Kenneth LiuSteven Bozinovski
Apr 15, 2008·Trends in Pharmacological Sciences·John M HallettAdriano G Rossi
Jun 24, 2008·The Journal of Clinical Investigation·Kai KessenbrockDieter E Jenne
Mar 25, 2009·Antioxidants & Redox Signaling·Vincent JaquetJ David Lambeth
Apr 1, 2009·Antioxidants & Redox Signaling·Brian GriffithVictor J Thannickal
Apr 11, 2009·Antioxidants & Redox Signaling·Gary M BokochDavide Gianni
Apr 24, 2009·The Journal of Biological Chemistry·Agnes W BootsAlbert van der Vliet
May 15, 2009·Antioxidants & Redox Signaling·Thomas L LetoTakehiko Ueyama
May 21, 2009·European Journal of Clinical Investigation·B KollerD Hartl
Jun 9, 2009·Clinical Reviews in Allergy & Immunology·Steven M Holland
Aug 25, 2009·Nature Medicine·Louise HeckerVictor J Thannickal
Nov 7, 2009·Mutagenesis·Nejla GüngörFrederik J van Schooten

❮ Previous
Next ❯

Citations

Dec 2, 2015·Experimental and Therapeutic Medicine·Yazhen WangGuofu Wang
Jul 29, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xue jun LvYu ying Li
Mar 22, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Nuria E Cabrera-BenítezJesús Villar
Jun 24, 2018·Clinical and Experimental Pharmacology & Physiology·Vandana R ThakurAnita A Mehta
May 17, 2014·The European Respiratory Journal·Erika TokitaBruce K Rubin
Jul 28, 2015·Chembiochem : a European Journal of Chemical Biology·Tarja KokkolaMaija Lahtela-Kakkonen
Feb 4, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Intae LeeAron B Fisher
Dec 10, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rebecca L OrndorffShampa Chatterjee
Mar 26, 2021·Frontiers in Pharmacology·Giselle Santos MagalhãesMaria José Campagnole-Santos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis