PMID: 9191028May 1, 1997Paper

Neutrophils in beige mice secrete normal amounts of cathepsin G and a 46 kDa latent form of elastase that can be activated extracellularly by proteolytic activity

Biological Chemistry
E CavarraG Lungarella

Abstract

Among other phenotypic defects, the beige mouse is susceptible to infection and has large neutrophil granules that apparently secrete a decreased amount of elastolytic activity. We have shown using in vitro methods that cytosolic inhibitors in beige neutrophils are normal. Although cathepsin G is tightly bound to lysosomal membranes, normal amounts of activity are released in response to degranulating agents. Decreased elastolytic activity is secreted by beige neutrophils because elastase is present in the granules as a 46 kDa proenzyme, which can be activated extracellularly by a protease-dependent mechanism. The current experiments were undertaken to explore the in vivo functions of neutrophils from C57 BI/6J (bg/bg) beige mice using the model of casein-induced acute peritonitis; normal C57 BI/6J (+/+) mice served as controls. The kinetics of neutrophil accumulation in the peritoneum were normal, suggesting normal neutrophil migration. Cathepsin G activity in the cell-free supernatant of peritoneal lavage fluid was normal; elastolytic activity was initially very low but increased to about twice baseline level after 4 h at 25 degrees C and to about 20-fold at 36 h. The appearance of this activity was inhibited to varying degre...Continue Reading

References

Jul 1, 1979·The Journal of Clinical Investigation·R A HaakL A Boxer
Apr 1, 1978·The Journal of Experimental Medicine·J D VassalliE Reich
Jun 1, 1991·The American Review of Respiratory Disease·B Starcher, H James
Aug 1, 1989·Experimental and Molecular Pathology·M M de SantiG Lungarella
Sep 1, 1989·Experimental Lung Research·B Starcher, I Williams
Sep 1, 1988·The Journal of Experimental Medicine·R J JohnsonS J Klebanoff
Jan 1, 1986·The American Review of Respiratory Disease·G L SniderP J Stone
Jan 1, 1985·Annual Review of Medicine·A Janoff
Jan 1, 1968·Advances in Immunology·C G Cochrane
Nov 1, 1967·The Journal of Heredity·M A LutznerH W Jordan
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen
Jan 1, 1981·Methods in Enzymology·A J Barrett
Jul 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·V UmanskyM Rocha
Oct 1, 1996·Archives of Disease in Childhood·S E DaleyK Bartlett
Jan 1, 1995·Connective Tissue Research·B Starcher, M Conrad

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Citations

Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·E CavarraG Lungarella
Jan 29, 2013·Hematology/oncology Clinics of North America·Marshall S HorwitzTimothy Tidwell
Dec 9, 2008·Pharmacology & Therapeutics·Christopher M Evans, Ja Seok Koo
Aug 9, 2005·Pulmonary Pharmacology & Therapeutics·G G BrusselleR A Pauwels
Apr 17, 2007·Human Mutation·Stephen J SalipanteMarshall S Horwitz

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