Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro

Frontiers in Immunology
Okan W BastianTaco J Blokhuis

Abstract

Although controlled local inflammation is essential for adequate bone regeneration, several studies have shown that hyper-inflammatory conditions after major trauma are associated with impaired fracture healing. These hyper-inflammatory conditions include the trauma-induced systemic inflammatory response to major injury, open fractures, and significant injury to the surrounding soft tissues. The current literature suggests that increased or prolonged influx of neutrophils into the fracture hematoma may mediate impairment of bone regeneration after hyper-inflammatory conditions. The underlying mechanism remains unclear. We hypothesize that high neutrophil numbers inhibit synthesis of mineralized extracellular matrix (ECM) by bone marrow stromal cells (BMSCs). We therefore studied the effect of increasing concentrations of neutrophils on ECM synthesis by human BMSCs in vitro. Moreover, we determined how high neutrophil concentrations affect BMSC cell counts, as well as BMSC osteogenic activity determined by alkaline phosphatase (ALP) expression and ALP activity. Co-culture of BMSCs with neutrophils induced a 52% decrease in BMSC cell count (p < 0.01), a 64% decrease in the percentage of ALP+ cells (p < 0.001), a 28% decrease in t...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D B DubravecM L Rodrick
Jul 1, 1991·The Journal of Cell Biology·K Turksen, J E Aubin
Sep 1, 1990·The Journal of Bone and Joint Surgery. British Volume·K MizunoK Hirohata
Jan 1, 1990·Archives of Orthopaedic and Trauma Surgery·B GrøgaardO Reikerås
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Jun 1, 1993·Acta Orthopaedica Scandinavica·O Grundnes, O Reikerås
Jun 1, 1997·Immunology Letters·P J Coffer, L Koenderman
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sang-Hyun ParkJay R Lieberman
Dec 29, 2007·The Journal of Pathology·X Zhang, D M Mosser
Jan 29, 2008·Injury·Peter V GiannoudisDavid Marsh
Jul 26, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ryan M PorterChristopher H Evans
Dec 17, 2008·Clinical and Experimental Immunology·E Fortunativan J M M Van den Bosch
Dec 25, 2008·Annual Review of Immunology·Fernando O MartinezSiamon Gordon
Jul 1, 2010·Thrombosis and Haemostasis·Leo KoendermanLaurien H Ulfman
Jan 7, 2011·Journal of Leukocyte Biology·Okan BastianTaco Blokhuis
Feb 10, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kylie A AlexanderAllison R Pettit
Mar 26, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan RecknagelAnita Ignatius
Apr 6, 2011·The Journal of Bone and Joint Surgery. British Volume·G CoxP V Giannoudis
Aug 13, 2011·Biomaterials·Omar M OmarPeter Thomsen
Aug 24, 2011·Tissue Engineering. Part a·Debby GawlittaWouter J A Dhert
Sep 17, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan RecknagelAnita Ignatius
Dec 14, 2011·The Journal of Clinical Investigation·Janesh PillayLeo Koenderman
Feb 2, 2012·Nature Reviews. Rheumatology·Lutz ClaesAnita Ignatius
Jan 29, 2013·The Journal of Trauma and Acute Care Surgery·Stefan RecknagelAnita Ignatius
Apr 30, 2013·Journal of Leukocyte Biology·Tamar TakLeo Koenderman
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jia ChangCun-Yu Wang
Feb 1, 2012·Advances in Wound Care·Christopher J Ferrante, Samuel Joseph Leibovich
Nov 7, 2014·Frontiers in Immunology·Cristina TecchioMarco A Cassatella
Nov 4, 2015·Bone·Claudia SchlundtKatharina Schmidt-Bleek
Feb 9, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Okan W BastianTaco J Blokhuis
Jun 9, 2016·Journal of Inflammation Research·Okan W BastianTaco J Blokhuis
Jul 28, 2016·European Cells & Materials·A KovtunA Ignatius
Apr 1, 2017·Cell and Tissue Research·Yang ZhangJeroen J J P van den Beucken
Nov 25, 2017·Frontiers in Immunology·Pien HellebrekersLeo Koenderman

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Citations

Jul 20, 2019·Regenerative Medicine·Venkata P MantripragadaGeorge F Muschler
Jul 23, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michiel CroesH Charles Vogely
Sep 12, 2020·Advanced Materials·Saber Amin YavariMichiel Croes
Oct 20, 2019·International Journal of Molecular Sciences·Gaurav Kumar, Pierre-Marie Roger
May 25, 2021·Seminars in Cell & Developmental Biology·Verena Fischer, Melanie Haffner-Luntzer

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Methods Mentioned

BETA
flow cytometry
flowcytometry
Fluorescence-activated cell sorting
FACS
fluorescence-activated cell sorter
light microscopy
fluorescence microscopy
Assay

Software Mentioned

GraphPad Prism
FlowJo
GraphPad

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