Neutrophils injure gallbladder interstitial Cajal-like cells in a guinea pig model of acute cholecystitis

Journal of Cellular Physiology
Meng-Juan LinBao-Ping Yu

Abstract

Acute cholecystitis is a common disease with gallbladder dysmotility. Disease pathogenesis involves immune cell infiltration as well as changes in gallbladder interstitial Cajal-like cells (ICLCs). However, it remains unclear if or how the immune cells affect ICLC morphology, density, distribution, and function in gallbladder tissue during acute cholecystitis. In this study, we explored the acute cholecystitis-related alterations in gallbladder ICLCs in a guinea pig model, focusing on the effects of neighboring neutrophils. Adult guinea pigs were randomly divided into four groups (control, 24 hr common bile duct ligation [CBDL], 48-hr CBDL, and antipolymorphonuclear neutrophil [PMN] treated) and analyzed using methylene blue staining and immunofluorescence. Gallbladder contractility was also monitored. To culture gallbladder ICLCs, collagenase digestion was performed on tissue from 10- to 15-day-old guinea pigs. Neutrophils isolated from the peripheral blood of experimental animals 48-hr postsurgery were also cocultured with the gallbladder ICLCs. Intracellular calcium was detected with Fluo-4 AM dye. Our results showed that gallbladder ICLC density significantly declined during acute cholecystitis and was accompanied by shorte...Continue Reading

References

Aug 2, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Peter R StregeGianrico Farrugia
Mar 24, 2005·Journal of Cellular and Molecular Medicine·L M PopescuCarmen Ardelean
Jun 21, 2005·Journal of Cellular and Molecular Medicine·Sanda M CionteaL M Popescu
Mar 28, 2006·Journal of Cellular and Molecular Medicine·M E HinescuL M Popescu
Jun 27, 2006·Journal of Cellular and Molecular Medicine·G P SergeantM A Hollywood
Jul 29, 2006·The Journal of Physiology·Kenton M Sanders, Sean M Ward
Sep 23, 2006·Journal of Cellular and Molecular Medicine·Jing YeJan D Huizinga
Sep 23, 2006·Scandinavian Journal of Gastroenterology·Xiaomin SunHesheng Luo
Jun 27, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K M ChoiG Farrugia
May 6, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C d'antonioJ D Huizinga
Sep 26, 2009·Gastroenterology·Jan D HuizingaGianrico Farrugia
Apr 7, 2010·Journal of Cellular and Molecular Medicine·L M Popescu, Maria-Simonetta Faussone-Pellegrini
Jul 9, 2010·World Journal of Gastroenterology : WJG·Randa-M MostafaHosam Hamdy
Dec 17, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Mihnea I NicolescuLaurentiu M Popescu
Nov 13, 2014·Journal of Cellular and Molecular Medicine·Xiao-Jun YangZong-Ji Shen
Oct 8, 2015·The Journal of Surgical Research·Peter C AmbeHubert Zirngibl
Jan 26, 2016·Folia morphologica·A PasternakA Matyja
May 11, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhen-Peng HuangBao-Ping Yu
Nov 9, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhen-Peng HuangBao-Ping Yu

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Citations

Sep 17, 2020·Expert Review of Gastroenterology & Hepatology·Yingyu LiuShuodong Wu
May 29, 2021·World Journal of Clinical Cases·Bei-Bei FuYing Fan

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