New abundant microbial groups in aquatic hypersaline environments.

Scientific Reports
Rohit GhaiF Rodríguez-Valera

Abstract

We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundant and previously unsuspected microbial groups. We describe for the first time, a group of low GC Actinobacteria, related to freshwater Actinobacteria, abundant in low and intermediate salinities. Metagenomic assembly revealed three new abundant microbes: a low-GC euryarchaeon with the lowest GC content described for any euryarchaeon, a high-GC euryarchaeon and a gammaproteobacterium related to Alkalilimnicola and Nitrococcus. Multiple displacement amplification and sequencing of the genome from a single archaeal cell of the new low GC euryarchaeon suggest a photoheterotrophic and polysaccharide-degrading lifestyle and its relatedness to the recently described lineage of Nanohaloarchaea. These discoveries reveal the combined power of an unbiased metagenomic and single cell genomic approach.

References

May 19, 2000·FEMS Microbiology Ecology·C Pedrós-AlióN Guixa-Boixereu
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Jul 6, 2000·Applied and Environmental Microbiology·J AntónR Amann
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Mar 17, 2001·Microbial Ecology·S. BenllochF. Rodríguez-Valera
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·D R ArahalA Ventosa
Apr 5, 2002·International Journal of Systematic and Evolutionary Microbiology·Josefa AntónRamón Rosselló-Mora
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Frank B DeanRoger S Lasken
Jun 20, 2002·Environmental Microbiology·Susana BenllochFrancisco Rodríguez-Valera
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Nov 6, 2004·Applied and Environmental Microbiology·Kathleen A PageStephen J Giovannoni
Jun 4, 2005·Applied and Environmental Microbiology·Arumugham RaghunathanRoger S Lasken
Mar 4, 2006·Microbiology·Jörg Soppa
May 30, 2006·Nature Biotechnology·Kun ZhangGeorge M Church
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ramunas Stepanauskas, Michael E Sieracki
Sep 20, 2007·PloS One·Ana-Belen Martín-CuadradoFrancisco Rodríguez-Valera
Dec 14, 2007·Genome Research·Victor KuninPhilip Hugenholtz
Oct 23, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hideki NoguchiTakehiko Itoh
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Feb 27, 2009·International Journal of Systematic and Evolutionary Microbiology·María ArenasJuan Soliveri
Apr 25, 2009·PloS One·Tanja WoykeRamunas Stepanauskas
Oct 17, 2009·Nature Reviews. Microbiology·Francisco Rodriguez-ValeraAlex Mira
Oct 27, 2009·International Journal of Systematic and Evolutionary Microbiology·Shu-Kun TangWen-Jun Li
Feb 12, 2010·The ISME Journal·Beltran Rodriguez-BritoForest Rohwer
May 21, 2010·Environmental Microbiology·Matthew E RhodesChristopher H House
Jun 22, 2010·International Journal of Systematic and Evolutionary Microbiology·Cristina Sánchez-PorroAntonio Ventosa
Oct 22, 2010·The ISME Journal·Jane L HeywoodRamunas Stepanauskas
Jan 29, 2011·Science·Matthias HessEdward M Rubin
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Ryan J NewtonStefan Bertilsson
May 10, 2011·Science·Hwan Su YoonDebashish Bhattacharya
Feb 1, 2012·Environmental Microbiology Reports·Rohit GhaiFrancisco Rodriguez-Valera

❮ Previous
Next ❯

Citations

Aug 22, 2012·Extremophiles : Life Under Extreme Conditions·Rahel Elevi Bardavid, Aharon Oren
Jan 30, 2013·Extremophiles : Life Under Extreme Conditions·Olga ZhaxybayevaR Thane Papke
Sep 17, 2013·Nature Communications·Lesley A OgilvieBrian V Jones
Aug 21, 2012·Applied and Environmental Microbiology·Ines BoujelbenFernando Santos
Jul 3, 2013·Annual Review of Microbiology·Pierre OffreChrista Schleper
Nov 22, 2013·BioMed Research International·Sofia Nikolaki, George Tsiamis
Nov 15, 2013·BMC Genomics·Mario López-PérezFrancisco Rodriguez-Valera
Sep 7, 2012·Aquatic Biosystems·Polpass Arul Jose, Solomon Robinson David Jebakumar
Apr 6, 2012·PloS One·Inmaculada Garcia-HerediaFrancisco Rodriguez-Valera
May 3, 2013·PloS One·Sheila PodellEric E Allen
Apr 1, 2014·Extremophiles : Life Under Extreme Conditions·Loubna TaziKeith A Crandall
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Aharon Oren
Aug 19, 2014·Extremophiles : Life Under Extreme Conditions·Antonio VentosaFrancisco Rodriguez-Valera
Aug 13, 2014·Extremophiles : Life Under Extreme Conditions·Brian P HedlundTanja Woyke
Aug 15, 2014·Nature Communications·Manuel Martínez-GarcíaJosefa Antón
Aug 27, 2014·Extremophiles : Life Under Extreme Conditions·Dimitry Y SorokinGerard Muyzer
May 23, 2015·Life·R Thane PapkeAntonio Ventosa
Nov 18, 2014·Life·Alison W S LukRicardo Cavicchioli
Nov 6, 2015·International Journal of Systematic and Evolutionary Microbiology·Paulina CorralAntonio Ventosa
Sep 24, 2015·International Journal of Systematic and Evolutionary Microbiology·María José LeónJosé Luis Copa-Patiño
Mar 3, 2015·Current Opinion in Biotechnology·Aharon Oren
Jun 24, 2015·Research in Microbiology·Cyrielle GascPierre Peyret
Mar 10, 2016·Extremophiles : Life Under Extreme Conditions·Yannick Y CorsellisCécile C Militon
Jul 8, 2015·Biochimie·Nina S AtanasovaHanna M Oksanen
Jun 7, 2015·Current Opinion in Microbiology·D A CowanP De Maayer
Jun 5, 2015·International Journal of Systematic and Evolutionary Microbiology·Carmen Infante-DomínguezAntonio Ventosa
May 2, 2015·The ISME Journal·Adrian-Ştefan AndreiHoria Leonard Banciu
Dec 7, 2014·International Journal of Systematic and Evolutionary Microbiology·Carmen Infante-DominguezAntonio Ventosa
Feb 11, 2015·International Journal of Systematic and Evolutionary Microbiology·Carmen Infante-DomínguezAntonio Ventosa
Nov 13, 2014·Environmental Microbiology·María GomarizJosefa Antón
Apr 30, 2015·Systematic and Applied Microbiology·Brian P HedlundJames T Staley
Jun 27, 2015·Current Opinion in Microbiology·Brian P HedlundTanja Woyke
Jun 10, 2015·Current Opinion in Microbiology·Antonio VentosaR Thane Papke
May 2, 2015·Current Opinion in Microbiology·Nina S AtanasovaDennis H Bamford
Oct 3, 2012·Current Opinion in Microbiology·Ramunas Stepanauskas
Aug 14, 2015·BMC Genomics·Ana-Belen Martin-CuadradoFrancisco Rodriguez-Valera

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP007685
AGNT00000000

Methods Mentioned

BETA
PCR
fluorescence-activated cell-sorting
phylogenetic profile
MDA
PCRs
single

Software Mentioned

BLASTP
sff
R package FactoMineR
Tcoffee
EMBOSS
PhyML
iep
MUSCLE
extract
BLASTN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.