New alterations of serum glycoproteins in alcoholic and cirrhotic patients revealed by high resolution two-dimensional gel electrophoresis

Biochemical and Biophysical Research Communications
P GravelJ Guimon

Abstract

Plasma protein are synthesized and secreted by the liver. Several reports have shown that excessive consumption of ethanol interferes with the hepatic protein synthesis and/or secretion. This study was undertaken to identify the plasma/serum proteins altered in two groups of patients with different alcohol-related diseases: actively drinking alcoholic patients group without liver disease and alcohol cirrhotic patients group. Two-dimensional gel electrophoresis was used to separate proteins with high resolution. Proteins were detected by silver staining and glycoproteins were specifically visualized and analyzed after lectin blotting followed by chemiluminescence detection. Different protein alterations were identified in each group of patients. In the alcoholic group, two new glycosylation modifications of serum proteins were identified. An abnormal microheterogeneity of haptoglobin and alpha1-antitrypsin was detected in the serum of all alcoholic patients. We also characterized by two-dimensional gel electrophoresis the carbohydrate deficient transferrin. The modifications of haptoglobin, alpha1-antitrypsin and transferrin present a similar change of charge and molecular weight in the two-dimensional gel electrophoresis patter...Continue Reading

Citations

Apr 12, 2012·Glycobiology·Lori Sc Kreisman, Brian A Cobb
Jan 7, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·D F Hochstrasser
Aug 2, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Peter B LuppaJochen Metzger
May 13, 2006·Glycoconjugate Journal·Sunil K RaghavHasi R Das
Feb 14, 2012·Journal of Proteomics·S BekesovaR Goldman
Mar 23, 2010·Trends in Molecular Medicine·Matthew L CowanSanjeev Krishna
Feb 20, 2009·Journal of Viral Hepatitis·K J CheungH Van Vlierberghe
Mar 21, 2006·Biochemical and Biophysical Research Communications·Zhicong HeBrett Garner
Jan 23, 2009·Journal of Hepatology·Bram BlommeHans Van Vlierberghe
May 2, 2008·The International Journal of Biochemistry & Cell Biology·Arancha Botella-LopezJavier Sáez-Valero
Oct 18, 2016·Alcoholism, Clinical and Experimental Research·Carol A CaseyArmen Petrosyan
Aug 1, 2007·Proteomics. Clinical Applications·Roisean E FergusonRosamonde E Banks
Apr 12, 2008·European Journal of Gastroenterology & Hepatology·Kin Jip CheungHans Van Vlierberghe
Jul 16, 2008·Journal of Separation Science·Milan MaderaMilos V Novotny
May 17, 2005·Expert Review of Proteomics·Gert Van den Bergh, Lutgarde Arckens
Jun 25, 1998·Electrophoresis·N H PackerA A Gooley
Feb 27, 2010·European Journal of Gastroenterology & Hepatology·Kin Jip CheungHans Van Vlierberghe
Feb 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Salud García-AyllónJavier Sáez-Valero
Oct 6, 2005·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·M Johanna LiinamaaMarkku J Savolainen
May 6, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sean O RyanBrian A Cobb
Dec 18, 2013·The Analyst·Chen-Chun ChenRofeamor P Obena
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
May 31, 2011·European Journal of Gastroenterology & Hepatology·Kin Jip CheungHans Van Vlierberghe
Feb 6, 2013·International Journal of Molecular Medicine·Kazushi SugimotoKazuhiko Uchida
Jun 21, 2013·Electrophoresis·Kala JessieOnn Haji Hashim
Sep 24, 2018·Molecular Biology Reports·Abo El-Khair B El-SayedMohamed M Seif
Nov 4, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kaori MochizukiIrwin M Arias
Apr 18, 2009·Journal of Proteome Research·Henning RosenfeldAndreas Prange
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·S Kishino, K Miyazaki
Jun 17, 1999·Annals of Clinical Biochemistry·G KeirP Clayton
May 3, 2005·Journal of Chromatography. a·Ziping Yang, William S Hancock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.