New antigenic candidates in multiple sclerosis: identification by serological proteome analysis

Proteomics
L AlmerasLionel Prin

Abstract

Myelin antigen targets that are clearly associated with pathogenic events in multiple sclerosis (M.S.) patients remain to be defined. We recently demonstrated that the analysis of global IgG antibody response against human brain antigens using one-dimensional (1-D) immunoblotting, allowed us to discriminate M.S. patients from controls (both healthy subjects and patients with Sjögren's syndrome). Additionally, this approach also differentiated the three clinical forms of M.S. Indeed, 42 brain antigenic bands (26 from healthy brain and 16 from the M.S. brain) showed the discriminant IgG immune responses. The aim of our study was to characterize the 26 discriminant antigenic bands detected in healthy brain. Protein identification was successively performed by 1-D and two-dimensional (2-D) immunoblottings using sera from 18 M.S. patients, followed by mass spectrometry (MS) analysis and a database search. One hundred and two antigenic spots were then detected on 2-D immunoblots, with M.S. sera against healthy brains. Sixty-four spots were successfully matched with 2-D Coomassie brillant blue stained gels, which were further selected for MS analysis and annotated leading to the identification of 14 of the 26 discriminant antigens. Th...Continue Reading

Citations

Jul 12, 2011·Parasitology Research·Albin FontaineLionel Almeras
Mar 7, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Daniela PriviteraRaffaella Fazio
Aug 24, 2005·Médecine sciences : M/S·Nadine MachourRoland Charlionet
Jun 5, 2013·Molecular & Cellular Proteomics : MCP·Burcu AyogluPeter Nilsson
May 27, 2010·Malaria Journal·Sébastien BriolantBruno Pradines
Jul 23, 2013·PloS One·Christophe FraisierLionel Almeras
Jun 1, 2007·Biomarkers in Medicine·Charlotte E TeunissenGavin Giovannoni
May 14, 2005·Current Opinion in Neurology·Saleh M Ibrahim, Ralf Gold
Apr 11, 2012·Current Opinion in Neurology·Tobias Derfuss, Edgar Meinl
Feb 24, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Oliver W GramlichFranz H Grus
Dec 18, 2014·International Journal of Molecular Sciences·Paolo ColombaGiovanni Duro
Jul 23, 2015·Proteomics. Clinical Applications·Antoine MénoretAnthony T Vella
Apr 7, 2007·Journal of Neuropathology and Experimental Neurology·Ruth HuizingaSandra Amor
Jan 19, 2012·Interdisciplinary Perspectives on Infectious Diseases·Romuald DasséLionel Prin
Jul 26, 2005·Proteomics·Lynne ThadikkaranJean-Daniel Tissot
Dec 8, 2010·Proteomics. Clinical Applications·Archana M JastorffChristoph W Turck
Jun 21, 2006·Proteomics·Wolfgang Hueber, William H Robinson
Nov 30, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jean-Baptiste ChansonJérôme de Seze
Dec 18, 2013·Proteomics·Alessandro S FariasDaniel Martins-de-Souza
Jul 30, 2005·Journal of Neuroimmunology·Francesco LolliAnna Maria Papini
Aug 12, 2014·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M T WallinS Jacobson
Feb 8, 2011·Biochimica Et Biophysica Acta·Laurie BroadwaterJennifer McDonough
Dec 29, 2004·Lancet Neurology·Charlotte E TeunissenChris Polman
Mar 31, 2018·Journal of Cellular and Molecular Medicine·Xinlu WangShiwu Zhang
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veerle SomersPiet Stinissen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.