New approach to isolate mesenchymal stem cell (MSC) from human umbilical cord blood

Cell Biology International
Issam HussainMohamed El-Sheemy

Abstract

HUCB (human umbilical cord blood) has been frequently used in clinical allogeneic HSC (haemopoietic stem cell) transplant. However, HUCB is poorly recognized as a rich source of MSC (mesenchymal stem cell). The aim of this study has been to establish a new method for isolating large number of MSC from HUCB to recognize it as a good source of MSC. HUCB samples were collected from women following their elective caesarean section. The new method (Clot Spot method) was carried out by explanting HUCB samples in mesencult complete medium and maintained in 37°C, in a 5% CO2 and air incubator. MSC presence was established by quantitative and qualitative immunophenotyping of cells and using FITC attached to MSC phenotypic markers (CD29, CD73, CD44 and CD105). Haematopoietic antibodies (CD34 and CD45) were used as negative control. MSC differentiation was examined in neurogenic and adipogenic media. Immunocytochemistry was carried out for the embryonic markers: SOX2 (sex determining region Y-box 2), OLIG-4 (oligodendrocyte-4) and FABP-4 (fatty acid binding protein-4). The new method was compared with the conventional Rosset Sep method. MSC cultures using the Clot Spot method showed 3-fold increase in proliferation rate compared with conv...Continue Reading

References

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Citations

Jul 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·C W ChanH C Man
Jul 28, 2012·Dental Clinics of North America·Reuben H KimMo K Kang
Jul 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·C W ChanH C Man
May 13, 2014·Cell Transplantation·Tzu-Min ChanShinn-Zong Lin
May 14, 2017·Journal of Cellular Physiology·Harish C ChandramoorthyMohamed A Haidara

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