New approaches for enhanced detection of enteroviruses from Hawaiian environmental waters.

PloS One
Christina ConnellYuanan Lu

Abstract

Health risks associated with sewage-contaminated recreational waters are of important public health concern. Reliable water monitoring systems are therefore crucial. Current recreational water quality criteria rely predominantly on the enumeration of bacterial indicators, while potentially dangerous viral pathogens often remain undetected. Human enteric viruses have been proposed as alternative indicators; however, their detection is often hindered by low viral concentrations present in the environment. Reported here are novel and effective laboratory protocols for viral concentration and highly sensitive and optimized RT-PCR for the efficient detection of enteroviruses, an important enteric virus subset, in Hawaiian environmental waters. Eighteen published enterovirus primer pairs were comparatively evaluated for detection sensitivity. The primer set exhibiting the lowest detection limit under optimized conditions, EQ-1/EQ-2, was validated in a field survey of 22 recreational bodies of water located around the island of Oahu, Hawaii. Eleven sites tested positive for enterovirus, indicating fecal contamination at these locations. As an additional means of viral concentration, shellfish were collected from 9 sample sites and sub...Continue Reading

References

Aug 1, 1994·Applied and Environmental Microbiology·D N LeesW J Doré
May 1, 1993·Applied and Environmental Microbiology·M AbbaszadeganI L Pepper
Aug 1, 1994·Applied and Environmental Microbiology·M PuigR Girones
Jul 1, 1996·The Pediatric Infectious Disease Journal·L P NielsenH A Rotbart
Oct 1, 1996·Applied and Environmental Microbiology·H ChungM D Sobsey
Apr 1, 1996·Applied and Environmental Microbiology·K A ReynoldsI L Pepper
Jan 15, 2003·Clinical Microbiology Reviews·Dale W GriffinJoan B Rose
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Theng-Theng Fong, Erin K Lipp
Aug 9, 2005·Applied and Environmental Microbiology·Jed A FuhrmanRachel T Noble
Nov 5, 2005·Applied and Environmental Microbiology·Samuel Choi, Sunny C Jiang
Nov 28, 2007·Clinical Chemistry·Weston C HymasDavid R Hillyard
Jan 1, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Uta DierssenAlbert Heim
May 30, 2008·Current Opinion in Biotechnology·Albert BoschRosa M Pintó
Dec 25, 2010·Journal of Environmental Sciences (China)·Chongmiao ZhangDangcong Peng
Dec 1, 2008·Anthropology & Medicine·David B MenkesChrystal Jaye

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Citations

Dec 19, 2012·Current Opinion in Virology·Eva Theres Gensberger, Tanja Kostić
Jul 6, 2013·Journal of Virological Methods·Erin AllmannYuanan Lu
May 5, 2016·Medical Mycology·Nancy A ChowAnastasia P Litvintseva
Aug 17, 2018·MicrobiologyOpen·Lucia M C WerneckEdson Elias da Silva
Sep 25, 2019·International Journal of Environmental Research and Public Health·Xiaotong WenZhaokang Yuan
Jan 22, 2019·Reviews in Medical Microbiology : a Journal of the Pathological Society of Great Britain and Ireland·Antonio TonioloEle Ferrannini

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Methods Mentioned

BETA
PCR
electrophoresis
membrane filtration
nucleic acid extraction

Software Mentioned

BLAST
Basic Local Alignment Search Tool ( BLAST )

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