New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)

Structure
A MüllerU Heinemann

Abstract

Adrenodoxin (Adx) is a [2Fe-2S] ferredoxin involved in steroid hormone biosynthesis in the adrenal gland mitochondrial matrix of mammals. Adx is a small soluble protein that transfers electrons from adrenodoxin reductase (AR) to different cytochrome P450 isoforms where they are consumed in hydroxylation reactions. A crystallographic study of Adx is expected to reveal the structural basis for an important electron transfer reaction mediated by a vertebrate [2Fe-2S] ferredoxin. The crystal structure of a truncated bovine adrenodoxin, Adx(4-108), was determined at 1.85 A resolution and refined to a crystallographic R value of 0.195. The structure was determined using multiple wavelength anomalous dispersion phasing techniques, making use of the iron atoms in the [2Fe-2S] cluster of the protein. The protein displays the compact (alpha + beta) fold typical for [2Fe-2S] ferredoxins. The polypeptide chain is organized into a large core domain and a smaller interaction domain which comprises 35 residues, including all those previously determined to be involved in binding to AR and cytochrome P450. A small interdomain motion is observed as a structural difference between the two independent molecules in the asymmetric unit of the crysta...Continue Reading

References

Nov 15, 1977·The Biochemical Journal·R CammackL J Rogers
May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Nov 16, 1992·Biochemical and Biophysical Research Communications·H UhlmannR Bernhardt
Oct 5, 1992·Journal of Molecular Biology·A MargK Ruckpaul
Mar 1, 1992·Archives of Biochemistry and Biophysics·J L OmdahlW J Driscoll
Jan 1, 1991·Methods in Enzymology·F S Millett, L M Geren
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 1, 1988·Archives of Biochemistry and Biophysics·N SakihamaY Ichikawa
Dec 15, 1986·FEBS Letters·A HiwatashiY Ichikawa
Apr 15, 1972·Biochimica Et Biophysica Acta·K SuharaM Katagiri
Jan 1, 1996·Reviews of Physiology, Biochemistry and Pharmacology·R Bernhardt
Sep 1, 1996·Protein Science : a Publication of the Protein Society·T V BurovaW Pfeil
Feb 21, 1997·The Journal of Biological Chemistry·V Beckert, R Bernhardt
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Mar 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·S IkemizuK Fukuyama
Jan 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·V S Lamzin, K S Wilson
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Jan 23, 1987·Science·A T BrüngerM Karplus

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Citations

Jul 19, 2000·Proteins·A V GrinbergR Bernhardt
Jan 6, 2000·Archives of Biochemistry and Biophysics·I A PikulevaY Kim
Nov 22, 2001·Archives of Biochemistry and Biophysics·A V Grinberg, R Bernhardt
Mar 11, 2009·Journal of Biomolecular NMR·Xingfu XuMarcellus Ubbink
Nov 25, 2003·Biochemical and Biophysical Research Communications·Burkhard Schiffler, Rita Bernhardt
Jun 8, 2002·Journal of Molecular Biology·Chetlen R CrossnoeKurt L Krause
Nov 7, 2000·Progress in Biophysics and Molecular Biology·U HeinemannW Saenger
Jul 26, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Robert C Tuckey, Madeleine J Headlam
Dec 23, 2008·The Journal of Physical Chemistry. a·Derrick R J KollingSergei A Dikanov
Feb 13, 2003·European Journal of Biochemistry·Merche FaroCarlos Gómez-Moreno
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alex D SheftelRoland Lill
Jul 11, 2013·The Journal of Biological Chemistry·Robert YanAnnalisa Pastore
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Joachim Jose, Thomas F Meyer
Oct 7, 2011·Journal of Lipid Research·Walter L Miller, Himangshu S Bose
Nov 6, 2010·Endocrine Reviews·Walter L Miller, Richard J Auchus
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·I F SevrioukovaT L Poulos
Jan 13, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Germaine SainzYves Jouanneau
Nov 12, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Stephen G BellLuet-Lok Wong
Jun 8, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Toshio IwasakiTakashi Kumasaka
Jan 15, 1999·Endocrine Research·R BernhardtU Heinemann
Jan 16, 2007·Molecular and Cellular Endocrinology·Karl-Heinz StorbeckAmanda C Swart
Sep 24, 2004·Journal of Molecular Recognition : JMR·Miguel Prudêncio, Marcellus Ubbink
Jun 12, 2010·Biochimica Et Biophysica Acta·Kerstin Maria EwenRita Bernhardt
Nov 21, 2009·Journal of Inorganic Biochemistry·Anja BerwangerRita Bernhardt
Jul 29, 2009·Biochimica Et Biophysica Acta·Guillermina GoñiMilagros Medina
Feb 4, 2005·Biopolymers·Saurabh Kumar ShakyaVolkhard Helms
Apr 16, 2005·Journal of Cellular Biochemistry·Paola GhiselliniClaudio Nicolini
Aug 19, 2006·Biophysical Chemistry·Joachim BehlkeRita Bernhardt

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