New assay for measuring cell surface hydrophobicities of Candida dubliniensis and Candida albicans

Clinical and Diagnostic Laboratory Immunology
M A Jabra-RizkT F Meiller

Abstract

Hydrophobic interactions, based on cell surface hydrophobicity (CSH), are among the many and varied mechanisms of adherence deployed by the pathogenic yeast Candida albicans. Recently it was shown that, unlike C. albicans, C. dubliniensis is a species that exhibits an outer fibrillar layer consistent with constant CSH. Previously, C. dubliniensis grown at 25 or 37 degrees C was shown to coaggregate with the oral anaerobic bacterium Fusobacterium nucleatum. C. albicans, however, demonstrated similar coaggregation only when hydrophobic or grown at 25 degrees C. This observation implied that coaggregation of Candida cells with F. nucleatum is associated with a hydrophobic yeast cell surface. To test this hypothesis, 42 C. albicans and 40 C. dubliniensis clinical isolates, including a C. albicans hydrophobic variant, were grown at 25 and 37 degrees C and tested with the established hydrophobicity microsphere assay, which determines CSH levels based on the number of microspheres attached to the yeast cells. The coaggregation assay was performed in parallel experiments. All C. dubliniensis isolates grown at either temperature, hydrophobic 25 degrees C-grown C. albicans isolates, and the C. albicans hydrophobic variant, unlike the 37 ...Continue Reading

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Citations

Jul 20, 2010·Biotechnology Letters·Katty Goossens, Ronnie Willaert
Sep 29, 2007·Diagnostic Microbiology and Infectious Disease·María-Teresa BlancoAntonio-Cándido Gómez-García
Jun 6, 2009·Gerodontology·Roberta Diavana de SouzaEdvaldo Antonio Ribeiro Rosa
Oct 18, 2006·FEMS Yeast Research·Mary Ann Jabra-RizkTimothy Meiller
Oct 18, 2011·Micron : the International Research and Review Journal for Microscopy·Luciene C F PaivaMarcia E L Consolaro
May 21, 2013·Journal of Chemotherapy·Elisabetta VavalaLetizia Angiolella
Sep 19, 2001·Yeast

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