New Bacterial Phytase through Metagenomic Prospection

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nathálya FariasCarlos Meneses

Abstract

Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1968·Advances in Protein Chemistry·G N Ramachandran, V Sasisekharan
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Sep 1, 1995·Trends in Biochemical Sciences·R A Sayle, E J Milner-White
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jan 1, 1996·Letters in Applied Microbiology·L BielecS G Jackson
Feb 1, 1996·Applied and Environmental Microbiology·J ZhouJ M Tiedje
Jun 26, 1998·Microbiology·L J YankeK J Cheng
Dec 1, 1999·Journal of Microbiological Methods·H D BaeL B Selinger
Oct 25, 2000·Protein Science : a Publication of the Protein Society·A FiserA Sali
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jo Handelsman
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 16, 2015·Frontiers in Microbiology·Celia Méndez-GarcíaManuel Ferrer
Jul 30, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Paulo S MonteiroSebastião T de Rezende
Jan 7, 2016·Frontiers in Genetics·Alejandra Escobar-ZepedaAlejandro Sanchez-Flores
Feb 5, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Marc C DellerBernhard Rupp
Jun 28, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carlos MenesesDavid Johnston-Monje
Jun 20, 2017·Frontiers in Microbiology·Elizabeth T AloriOlubukola O Babalola
Jan 1, 2018·Plant and Soil·Daniel Menezes-BlackburnPhilip M Haygarth

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Citations

Jun 30, 2019·Applied Microbiology and Biotechnology·Kevin R HerrmannUlrich Schwaneberg
Mar 9, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Xiaoshen ZhaoHe Li
Jan 14, 2020··Asmita KambleHarinder Singh

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Datasets Mentioned

BETA
MG544855

Methods Mentioned

BETA
protein folding
size exclusion chromatography
Polymerase Chain
electrophoresis

Software Mentioned

ClustalW
PROCHECK
MEGA
CLUSTALX
RASMOL
MODELLER
BLAST ( Basic Local Alignment Search Tool
BLAST
ORF finder

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