New bioluminogenic substrates for monoamine oxidase assays

Journal of the American Chemical Society
Wenhui ZhouKeith Wood

Abstract

Novel bioluminogenic substrates were designed for probing monoamine oxidase (MAO) activity based on a simple and effective beta-elimination strategy. By modifying the amino group and the central core of luciferin derivatives, we have developed a series of substrates useful for assays of MAO A or B, or both. One of these substrates, exhibiting low Km values and high signal-to-background ratios with both isozymes, was shown to accurately measure the Ki values of known MAO inhibitors. This substrate is a key component in the development of a highly sensitive homogeneous MAO assay for high-throughput screening (HTS) of compounds in drug discovery and for monitoring MAO activity in complex biological systems. This design strategy should be applicable to fluorogenic MAO substrates and could broaden the structural requirements of substrates for other enzyme assays.

References

Dec 8, 2004·Analytical Biochemistry·Xiaoyun YangJoseph D Andrade
Apr 27, 2005·Brain Research. Brain Research Reviews·Silvia MandelMoussa B H Youdim
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Khalid ShahRalph Weissleder

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Citations

Dec 5, 2008·The Journal of Organic Chemistry·Ke-Qing Ling, Lawrence M Sayre
Nov 4, 2006·Chemical Communications : Chem Comm·Wenhui ZhouKeith V Wood
Nov 9, 2007·Chemical Communications : Chem Comm·Aaron E AlbersChristopher J Chang
Jul 2, 2009·Chemical Communications : Chem Comm·Qing ShaoBengang Xing
May 10, 2011·Chemical Communications : Chem Comm·Mi Eun JunKyo Han Ahn
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mark A SellmyerJennifer A Prescher
Mar 16, 2007·Assay and Drug Development Technologies·Frank Fan, Keith V Wood
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Satoshi Inouye
Dec 8, 2009·Current Opinion in Chemical Biology·Jennifer A Prescher, Christopher H Contag
Nov 7, 2006·Analytical Biochemistry·Michael P ValleyKeith V Wood
Apr 5, 2017·Journal of the American Chemical Society·Kevin J BruemmerChristopher J Chang
Jul 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rona R Ramsay, Keith F Tipton
Oct 27, 2017·Chemistry : a European Journal·Yuqi WangDeju Ye
Feb 14, 2015·Chemical Communications : Chem Comm·Roger H F WongHo Yu Au-Yeung
Feb 26, 2013·Chemistry : a European Journal·Jie ZhaoJunbai Li
Jun 13, 2012·Chembiochem : a European Journal of Chemical Biology·Mika ItoHiroshi Abe
Mar 5, 2014·Chembiochem : a European Journal of Chemical Biology·Wenhui ZhouPoncho Meisenheimer
Aug 7, 2007·Angewandte Chemie·Hequan YaoJianghong Rao
Feb 6, 2008·Chembiochem : a European Journal of Chemical Biology·Wenhui ZhouKeith V Wood
May 10, 2011·Chemical Society Reviews·Andrew RazgulinJianghong Rao
Jun 2, 2012·Chemical Communications : Chem Comm·Dokyoung KimKyo Han Ahn
Oct 27, 2012·Chemical Society Reviews·Jing LiMinyong Li
Mar 1, 2014·MedChemComm·Miranda A Paley, Jennifer A Prescher
May 20, 2014·Organic & Biomolecular Chemistry·Dokyoung KimKyo Han Ahn
Sep 6, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Poncho L MeisenheimerJames J Cali
Dec 17, 2008·Chemical Communications : Chem Comm·Jean-Louis ReymondNoélie Maillard
Jun 14, 2012·Chemical Communications : Chem Comm·Shaobo LongQing Zhu
Oct 10, 2013·Advanced Materials·Gulen Yesilbag TongaVincent M Rotello
Feb 21, 2014·Organic & Biomolecular Chemistry·Xuefeng LiQing Zhu

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