Apr 10, 2014

New biomarkers of coffee consumption identified by the non-targeted metabolomic profiling of cohort study subjects

PloS One
Joseph A RothwellClaudine Manach


Coffee contains various bioactives implicated with human health and disease risk. To accurately assess the effects of overall consumption upon health and disease, individual intake must be measured in large epidemiological studies. Metabolomics has emerged as a powerful approach to discover biomarkers of intake for a large range of foods. Here we report the profiling of the urinary metabolome of cohort study subjects to search for new biomarkers of coffee intake. Using repeated 24-hour dietary records and a food frequency questionnaire, 20 high coffee consumers (183-540 mL/d) and 19 low consumers were selected from the French SU.VI.MAX2 cohort. Morning spot urine samples from each subject were profiled by high-resolution mass spectrometry. Partial least-square discriminant analysis of multidimensional liquid chromatography-mass spectrometry data clearly distinguished high consumers from low via 132 significant (p-value<0.05) discriminating features. Ion clusters whose intensities were most elevated in the high consumers were annotated using online and in-house databases and their identities checked using commercial standards and MS-MS fragmentation. The best discriminants, and thus potential markers of coffee consumption, were ...Continue Reading

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  • References35
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Mentioned in this Paper

Metabolic Process, Cellular
Neuro-Oncological Ventral Antigen 2
Diabetes Mellitus, Non-Insulin-Dependent
DRUG Screen Quant Caffeine
Plant alkaloid

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