New CD44 splice variants associated with human breast cancers

Journal of Cellular Physiology
N Iida, Lilly Y W Bourguignon

Abstract

Changes in the CD44 variant (CD44v) isoforms on the cell surface have been correlated with tumor metastasis. In this study we have examined the expression of CD44 variant isoforms in human breast carcinoma samples by a variety of techniques including immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR), and nucleotide sequencing. Using RT-PCR, we have determined that normal human breast tissue contains primarily the CD44 epithelial (CD44E) form and very little CD44 standard (CD44s) form. However, metastatic breast carcinomas appear to overexpress both the CD44E and CD44s forms and also display multiple new species of CD44 variant isoforms. Histocytochemical staining using anti-CD44 antibody (recognizing a common determinant of the CD44 class of glycoproteins) confirms that the CD44 molecules are overexpressed and preferentially located in metastatic breast cancer tissues. Nucleotide sequencing analyses indicate that at least four new CD44 variant isoforms (i.e., displaying unique splicing via the insertion or the deletion of exons 7, 10, 11, and 14) may be closely associated with human metastatic breast cancers. These newly described CD44 variant isoforms may be useful for monitoring the progression of...Continue Reading

References

Oct 1, 1992·Molecular and Cellular Biology·L Y BourguignonG J Bourguignon
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
May 1, 1991·The Journal of Clinical Investigation·S JalkanenP Klemi
Dec 1, 1989·Immunology Today·B F HaynesS M Denning
Jun 1, 1986·The Journal of Cell Biology·L Y BourguignonK Balazovich
Feb 1, 1988·The Journal of Cell Biology·E L Kalomiris, L Y Bourguignon
Mar 1, 1994·Experimental Eye Research·M H ChaitinA M Brun-Zinkernagel
Feb 1, 1994·Journal of Cutaneous Pathology·N S Penneys, S Shapiro
Dec 4, 1993·Lancet·M C Latham, E A Frongillo
Aug 28, 1993·Lancet·S B FoxJ Fawcett
Mar 11, 1993·Nucleic Acids Research·C TölgH Ponta

❮ Previous
Next ❯

Citations

Jun 8, 2001·Journal of Hepatology·M A Della FaziaG Servillo
Apr 25, 2003·Critical Reviews in Oncology/hematology·Tracey A MartinWen G Jiang
Jul 1, 1997·Breast Cancer Research and Treatment·N GuriecF Oberling
Nov 20, 1998·Breast Cancer Research and Treatment·N L CooperJ T Emerman
Dec 10, 2002·Hybridoma and Hybridomics·S M DavernS J Kennel
Apr 1, 1997·Molecular Pathology : MP·Z Rudzki, S Jothy
Mar 1, 2000·Molecular Pathology : MP·S GoodisonD Tarin
Mar 28, 2013·Journal of Drug Delivery·Silvia ArpiccoElias Fattal
Dec 15, 2015·Expert Opinion on Drug Delivery·N Vijayakameswara RaoJae Hyung Park
Mar 6, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun LiYong-Mei Yin
Apr 2, 2010·The Journal of Investigative Dermatology·Sabine DammHelmut Schaider
Nov 10, 2007·The American Journal of Pathology·Allal OuhtitMadhwa H G Raj
Jun 10, 2009·The Laryngoscope·Steven J WangLilly Y W Bourguignon
Jul 1, 1997·Human Pathology·J S LyzakL Joseph
May 23, 2006·Biochimica Et Biophysica Acta·Th A ChristopoulosD H Vynios
Aug 13, 2015·BMC Bioinformatics·Theodore RomanRussell Schwartz
Apr 30, 2002·Experimental Cell Research·Robert SternBent Formby
Nov 25, 1998·Gynecologic Oncology·M KatsuraT Aono
Nov 20, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Zina Jeyapalan RutnamBurton B Yang
Oct 21, 1998·Experimental Eye Research·M H Chaitin, A M Brun-Zinkernagel
Oct 4, 2012·The American Journal of Pathology·Simona DonatelloAnn M Hopkins
Dec 8, 2014·Biochimica Et Biophysica Acta·Suresh RamakrishnaKwang-Hyun Baek
Dec 1, 1996·Japanese Journal of Cancer Research : Gann·T TakanoN Amino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.