New Developments in Pichia pastoris Expression System, Review and Update

Current Pharmaceutical Biotechnology
Roghayyeh BaghbanNaser Samadi

Abstract

Although Pichia pastoris is an outstanding host among conventional expression systems for production of recombinant proteins, a new interest has been emerged to this system due to the inherent advantages and new developments in this expression host. The potential for secretory and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate for the production of complex eukaryotic proteins. Several new developments have occurred in different areas related to P. pastoris expression system including hosts, vectors, glycosylation pattern and fermentation technology. Strain engineering using Crispr/Cas9 technology to produce human-like glycoproteins and protease deficient strains are two new areas of development with high importance. This review is dedicated to discuss the most important characteristics of P. pastoris with emphasis on new developments, especially in the field of glycoengineering, efficient expression vectors and promoters. New developments that occurred in the P. pastoris expression system converted this system to a versatile host for the production of complex proteins. This progress paved the way for several proteins to enter the clinical trials or industrial processes with t...Continue Reading

Citations

Jun 21, 2019·Journal of Cellular Biochemistry·Vahideh AhmadzadehHabib Zarredar
Jul 3, 2019·Advanced Materials·Arne BiesiekierskiCuie Wen
Feb 26, 2019·Molecular Biotechnology·Roghayyeh BaghbanMaryam Aria
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Nagesh K Tripathi, Ambuj Shrivastava
Aug 30, 2020·Viruses·Flávia Fonseca BagnoFlávio Guimarães da Fonseca
Sep 24, 2020·Biotechnology and Bioengineering·Feng GuoMin Jiang
Oct 20, 2020·Frontiers in Immunology·Hajer AounallahMichail Kotsyfakis
Dec 15, 2020·Biological Procedures Online·Roghayyeh BaghbanNosratollah Zarghami
Jan 23, 2021·Microbial Cell Factories·Michael Dare AsemoloyeLorenzo Pecoraro
Jan 29, 2021·Frontiers in Microbiology·Lukas BernauerAnita Emmerstorfer-Augustin
Jan 23, 2021·International Journal of Molecular Sciences·Mohamed Amine JmelMichalis Kotsyfakis

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