New Escherichia coli outer membrane proteins identified through prediction and experimental verification

Protein Science : a Publication of the Protein Society
Paola MaraniG von Heijne

Abstract

Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five-YftM, YaiO, YfaZ, CsgF, and YliI--and also provide preliminary data indicating that a sixth--YfaL--may be an outer membrane autotransporter.

Citations

May 28, 2010·Journal of Molecular Evolution·Sarah M Chiang, Herb E Schellhorn
Jan 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ashley A NenningerScott J Hultgren
Jul 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hammad NaveedJie Liang
Aug 12, 2008·Journal of Bacteriology·Torsten SterzenbachChristine Josenhans
Feb 26, 2008·Journal of Bacteriology·Louise BaarsJan-Willem de Gier
Jan 1, 2014·PloS One·Douglas F BrowningIan R Henderson
May 15, 2015·Protein Science : a Publication of the Protein Society·Leonid S Brown, Vladimir Ladizhansky
Feb 15, 2011·Journal of Molecular Biology·Mirjam M KlepschJan-Willem L de Gier
Aug 11, 2015·Protein Science : a Publication of the Protein Society·Jared KimHo Leung Ng
Mar 30, 2010·Environmental Microbiology·Charalampia-Georgia KoreaChristophe Beloin
Jun 5, 2015·BMC Biotechnology·Felipe MolinaJosé E Rebollo
Oct 2, 2007·Biochimica Et Biophysica Acta·Joel H Weiner, Liang Li
Dec 30, 2014·Frontiers in Microbiology·Ambre SalaPierre Genevaux
Oct 30, 2016·International Journal of Medical Microbiology : IJMM·Juraj BosákDavid Šmajs
Apr 21, 2007·Molecular & Cellular Proteomics : MCP·Samuel WagnerJan-Willem de Gier
Oct 3, 2018·Communications Biology·Shinya SugimotoYoshimitsu Mizunoe
Oct 26, 2007·Journal of Chemical Information and Modeling·M Michael GromihaMakiko Suwa

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