PMID: 2112409Jun 1, 1990Paper

New evidence for the dimeric nature of NADH:Q oxidoreductase in bovine-heart submitochondrial particles

Biochimica Et Biophysica Acta
Ronald van BelzenS P Albracht

Abstract

The initial velocity of NADH oxidation by bovine-heart submitochondrial particles was measured at pH 8.0 after pretreatment of these particles with different amounts of the inhibitor piericidine A together with 0.035 mM NADH. The amount of piericidine A required to fully inhibit the NADH oxidation activity extrapolated to exactly 1.0 per Fe-S cluster 2 of NADH:Q oxidoreductase. When no reducing equivalents from NADH were present during the pretreatment, this ratio was 1.2. The difference is explained by assuming that NADH:Q oxidoreductase binds piericidine A more effectively in the reduced state than in the oxidized state. It was also found that after Q10-extraction and reincorporation of submitochondrial particles, the amount of piericidine A required to fully inhibit the NADH oxidation activity of the particles increased with the amount of Q10 present during reincorporation. This is explained by assuming that binding of piericidine A, to the inhibitory site of NADH:Q oxidoreductase requires Q10. When 0.035 mM NADPH instead of NADH was present during the pretreatment of submitochondrial particles with piericidine A, the amount of inhibitor per cluster 2 required to fully inhibit the initial NADH-oxidation activity extrapolated...Continue Reading

References

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Citations

Dec 1, 1994·Journal of Molecular Recognition : JMR·J OvádiH O Spivey
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·M Finel
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·H Weiss, T Friedrich
Sep 1, 2007·Biochemistry·Vera G GrivennikovaAndrei D Vinogradov
Nov 1, 1992·European Journal of Biochemistry·R van BelzenS P Albracht
Jun 15, 1994·European Journal of Biochemistry·A M De Jong, S P Albracht
Aug 30, 1994·Biochimica Et Biophysica Acta·T P Singer, R R Ramsay
Jul 4, 1997·Biochimica Et Biophysica Acta·T V Zharova, A D Vinogradov
Jan 16, 1997·Biochimica Et Biophysica Acta·S P AlbrachtP de Jong
Feb 8, 1993·Biochimica Et Biophysica Acta·T Yagi
Jul 29, 1994·Biochimica Et Biophysica Acta·A M de JongS P Albracht
May 8, 1991·European Journal of Biochemistry·H WeissD Preis
Aug 1, 1992·Quarterly Reviews of Biophysics·J E Walker

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