New evidence that nuclear import of endogenous beta-catenin is LEF-1 dependent, while LEF-1 independent import of exogenous beta-catenin leads to nuclear abnormalities

Cell Biology International
K Kim, E D Hay

Abstract

The once accepted idea that LEF-1 transports beta-catenin into nuclei has recently been challenged by experiments using exogenous beta-catenin. Here, we investigated the effects of beta-catenin and LEF-1 on nuclear import of beta-catenin using different combinations of exogenous and endogenous molecules over longer lengths of time than previously studied. Nuclear beta-catenin is not detectable in corneal fibroblasts and epithelia or NIH 3T3 and MDCK cells. In LEF-1 transfections, we show that the B-box of LEF-1 is required to move cytoplasmic endogenous beta-catenin into the nuclei of such cells, proving that LEF-1 does transport endogenous beta-catenin into nuclei. Moreover, transfection of uveal melanoma cells with B-box deficient LEF-1 inhibits nuclear import of beta-catenin by endogenous LEF-1. However, the movement of overexpressed exogenous beta-catenin into nuclei is unaffected by the presence or absence of LEF-1 and forms abnormal nuclear aggregates that are a prelude to subsequent apoptosis. We conclude that nuclear transport of exogenous beta-catenin independently of LEF-1 has questionable physiological significance.

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Citations

Jul 4, 2002·Cell Biology International·Kwonseop KimElizabeth D Hay
Dec 18, 2003·The Journal of Clinical Investigation·Raghu Kalluri, Eric G Neilson
Apr 12, 2011·Clinical Reviews in Bone and Mineral Metabolism·Joseph P Bidwell, Fredrick M Pavalko
Oct 6, 2010·Advanced Drug Delivery Reviews·Jia-Ling Teo, Michael Kahn
Jun 7, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elizabeth D Hay
Jun 25, 2009·Traffic·Kylie M Wagstaff, David A Jans
Oct 16, 2007·International Journal of Cancer. Journal International Du Cancer·Peter J Van VeldhuizenSushanta K Banerjee
Mar 17, 2010·The Biochemical Journal·Jaswinder K Sethi, Antonio Vidal-Puig
Jan 1, 2013·Molecular Cell·Ana Chocarro-CalvoCustodia García-Jiménez
Apr 21, 2020·Frontiers in Physiology·Asmat Ullah KhanJingxing Dai

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