New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

Cryobiology
S JudyckaG J Dietrich

Abstract

The goal of this study was to develop a simple glucose-methanol extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. We tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender on post-thaw sperm motility. Sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1 M glucose in 15% methanol (GM) were compared to previously described Tris-sucrose-KCl in 10% - methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 30 min equilibration in GM extender before cryopreservation and 30 min of post-thaw storage were determined. The beneficial effect of the glucose for semen cryopreservation was related to its concentration with a quite narrow optimum of 0.1 to -0.15 M. The fertilization rates of frozen/thawed sperm were similar for both (TSKM and GM) tested extenders. The sperm motility and fertilization rate were not affected either by 30 min equilibration in GM extender or by 30 min of post-thaw storage. Our work indicates that the use a simple extender consisting of 0.1M glucose in 15% methanol can be a...Continue Reading

References

May 19, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A CiereszkoJ Glogowski
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Sayyed Mohammad Hadi AlaviMohammd Ali Akhoundzadeh
May 27, 2014·Reproductive Biology·Borys DzyubaOtomar Linhart
Aug 26, 2014·Reproductive Biology·Viktoriya Dzyuba, Jacky Cosson

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Citations

Dec 4, 2015·Animal Reproduction Science·Galina ProkopchukJacky Cosson
Oct 8, 2015·Animal Reproduction Science·Mohammad Sadegh AramliAshkan Banan
Mar 9, 2018·Reproduction, Fertility, and Development·V Gallego, J F Asturiano

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