New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order.

The Journal of Biological Chemistry
Moran KopelEhud Banin

Abstract

Ulvan is the main polysaccharide component of the Ulvales (green seaweed) cell wall. It is composed of disaccharide building blocks comprising 3-sulfated rhamnose linked to d-glucuronic acid (GlcUA), l-iduronic acid (IdoUA), or d-xylose (Xyl). The degradation of ulvan requires ulvan lyase, which catalyzes the endolytic cleavage of the glycoside bond between 3-sulfated rhamnose and uronic acid according to a β-elimination mechanism. The first characterized ulvan lyase was identified in Nonlabens ulvanivorans, an ulvanolytic bacterial isolate. In the current study, we have identified and biochemically characterized novel ulvan lyases from three Alteromonadales isolated bacteria. Two homologous ulvan lyases (long and short) were found in each of the bacterial genomes. The protein sequences have no homology to the previously reported ulvan lyases and therefore are the first representatives of a new family of polysaccharide lyases. The enzymes were heterologously expressed in Escherichia coli to determine their mode of action. The heterologous expressed enzymes were secreted into the milieu subsequent to their signal sequence cleavage. An endolytic mode of action was observed and studied using gel permeation chromatography and (1)H ...Continue Reading

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Citations

Feb 1, 2018·The Journal of Biological Chemistry·ThirumalaiSelvi UlaganathanMiroslaw Cygler
Nov 1, 2017·Nucleic Acids Research·Nicolas TerraponBernard Henrissat
Sep 30, 2017·Bioscience, Biotechnology, and Biochemistry·Chuan HeKouhei Ohnishi
Jun 8, 2018·The Journal of Biological Chemistry·ThirumalaiSelvi UlaganathanMiroslaw Cygler
Jul 10, 2019·Nature Chemical Biology·Lukas ReiskyJan-Hendrik Hehemann
Jun 28, 2018·Applied Microbiology and Biotechnology·Lukas ReiskyUwe T Bornscheuer
Jul 8, 2017·Biochemical and Biophysical Research Communications·Chuan He, Kouhei Ohnishi
Apr 24, 2021·Chembiochem : a European Journal of Chemical Biology·Marcus BäumgenUwe T Bornscheuer

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