New findings about iron oxide nanoparticles and their different effects on murine primary brain cells

International Journal of Nanomedicine
Jenni NeubertJana Glumm

Abstract

The physicochemical properties of superparamagnetic iron oxide nanoparticles (SPIOs) enable their application in the diagnostics and therapy of central nervous system diseases. However, since crucial information regarding side effects of particle-cell interactions within the central nervous system is still lacking, we investigated the influence of novel very small iron oxide particles or the clinically approved ferucarbotran or ferumoxytol on the vitality and morphology of brain cells. We exposed primary cell cultures of microglia and hippocampal neurons, as well as neuron-glia cocultures to varying concentrations of SPIOs for 6 and/or 24 hours, respectively. Here, we show that SPIO accumulation by microglia and subsequent morphological alterations strongly depend on the respective nanoparticle type. Microglial viability was severely compromised by high SPIO concentrations, except in the case of ferumoxytol. While ferumoxytol did not cause immediate microglial death, it induced severe morphological alterations and increased degeneration of primary neurons. Additionally, primary neurons clearly degenerated after very small iron oxide particle and ferucarbotran exposure. In neuron-glia cocultures, SPIOs rather stimulated the outg...Continue Reading

Citations

Dec 23, 2015·Neural Regeneration Research·Jenni Neubert, Anja U Bräuer
Oct 27, 2015·International Journal of Molecular Sciences·Ujwal S PatilDouglas B Chrisey
Feb 18, 2017·Environmental Science and Pollution Research International·Elena SheidaSvetlana Notova
Mar 27, 2021·Reviews on Environmental Health·Abbas Mohammadipour, Mahmoud Abudayyak

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Methods Mentioned

BETA
PCR
light microscopy
transfection
FCS
fluorescence microscopy

Software Mentioned

CellSens Dimension
Adobe Photoshop
ImageJ
Image - Based Tool for Counting Nuclei
ABI PRISM
MagnaFire

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