New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase

Journal of Biochemistry
T MoritaT Kimura

Abstract

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.

Citations

Aug 1, 1986·International Journal of Peptide and Protein Research·G Kokotos, C Tzougraki
Jan 1, 1985·Clinical and Experimental Hypertension. Part A, Theory and Practice·H OhashiC Kawai
Jan 1, 1981·Pflügers Archiv : European journal of physiology·K TomitaF Sakai
Jul 11, 1994·FEBS Letters·J Anders, M Kemme
Mar 10, 1998·Journal of Biomedical Materials Research·H NoguchiY Ikada
Apr 3, 1998·The Journal of Veterinary Medical Science·T Horimoto, Y Kawaoka
Jul 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dhaval N Gosalia, Scott L Diamond
Nov 19, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Masaki YamaguchiTadamichi Shimizu
Apr 23, 2013·Archives of Insect Biochemistry and Physiology·Shunsuke FurihataYoichi Hayakawa
Jan 1, 1981·Annals of the New York Academy of Sciences·G Claeson, L Aurell
Aug 1, 1986·The British Journal of Dermatology·K HashimotoK Yoshikawa
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·D A Triplett
Feb 15, 1986·Experientia·T KambaraS Shoji
Jan 1, 1996·Parasitology·B A FurmidgeD I Pritchard
Feb 12, 2005·Journal of Molecular Recognition : JMR·Yasuhiro NishiyamaSudhir Paul
Oct 15, 2005·Journal of Biomedical Materials Research. Part a·Zhong-Fang LaiYasunori Kitamoto
Jun 7, 2006·Chembiochem : a European Journal of Chemical Biology·Luke D LavisRonald T Raines
Oct 1, 2005·Chemistry : a European Journal·Shun-Ichi TamaruItaru Hamachi
Oct 26, 2010·Analytical and Bioanalytical Chemistry·Vanessa Castro-LópezAnthony J Killard
Dec 9, 2008·Bioscience, Biotechnology, and Biochemistry·Saori TakahashiSeihan Yamada
Jan 1, 1991·Hypertension·T YamaguchiA G Scicli
Jan 1, 1982·Clinical and Experimental Hypertension. Part A, Theory and Practice·T HataC A Nugent
Feb 15, 1988·European Journal of Biochemistry·S KawabataS Sakakibara
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa MaffioliGabriella Tedeschi
Mar 14, 1991·European Journal of Biochemistry·Y VannesteM Deschodt-Lanckman
Jul 1, 1994·Occupational and Environmental Medicine·P BoscoloG Giuliano
Sep 13, 2003·British Journal of Pharmacology·Wu YangSui Xiong Cai
Oct 12, 1996·Annals of the New York Academy of Sciences·X J CaoG C Dai
Mar 18, 2000·Biological Chemistry·H KolkenbrockN Ulbrich
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takahisa ImamuraKeinosuke Okamoto
Feb 12, 2010·The Biochemical Journal·Xiaoping FuGuanghua Zhao
Jul 29, 2016·PloS One·Shunsuke FurihataYoichi Hayakawa
May 1, 1992·Journal of Biomedical Materials Research·G Rollason, M V Sefton
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·K Ando, T Fujita
Oct 1, 1985·Archives of Disease in Childhood·M UchiyamaK Sakai
Jul 1, 1991·American Journal of Respiratory Cell and Molecular Biology·K TaniT Ogura
Sep 18, 1998·Journal of Biomedical Materials Research·Y SuzukiY Shimizu
May 1, 1986·The Annals of Otology, Rhinology & Laryngology. Supplement·Y HamaguchiY Sakakura
Aug 6, 2003·European Journal of Biochemistry·Hitoshi MatsumotoYoichi Hayakawa
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S VisvanathanH P McNeil
Feb 1, 1995·The Journal of Applied Bacteriology·H J OakeyK W Knox
Nov 12, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Farouc A JafferRalph Weissleder
Mar 28, 2009·Proceedings. Biological Sciences·Rachel I FlemingMalcolm W Kennedy
Feb 2, 2012·ChemMedChem·Sander S van BerkelFloris P J T Rutjes
Oct 22, 2014·Biomicrofluidics·Jiaqing YuChia-Hung Chen
Apr 1, 2006·Biotechnology and Bioengineering·Dhaval N GosaliaScott L Diamond
Sep 12, 2019·Pharmacology Research & Perspectives·Masako SasakiTomohiro Kawamoto
Dec 5, 2020·Future Medicinal Chemistry·Marianne E MareshDarci J Trader

Related Concepts

Metazoa
Coumarins
Factor X
Padutin
Pancreas
Endopeptidases
Structure-Activity Relationship
Substrate Specificity
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