New formats of polymeric stationary phases for HPLC separations: molded macroporous disks and rods

Journal of Molecular Recognition : JMR
F Svec, Jean M J Fréchet

Abstract

A molding process has been used for the preparation of separation media in different shapes such as rods and flat membrane-like disks. The polymerization is carried out using a mixture of monomers, porogenic solvent and free-radical initiator under conditions that afford macroporous materials with through-pores or channels large enough to provide the high flow characteristics required for applications in chromatography. In contrast to classical suspension polymerization, the solubility of monomers in water does not restrict their use. The versatility of the preparation technique is demonstrated in polymerizations involving both hydrophobic and hydrophilic monomers such as styrene, chloromethylstyrene, glycidyl methacrylate, alkyl methacrylates and acrylamide. Techniques have been developed that allow fine control of the porous properties of the polymers. These, in turn, determine the hydrodynamic properties of the separation devices that contain the molded media. Since all the mobile phase must flow through the separation medium, the mass transport within the molded media is accelerated considerably by convection. Therefore, the separations can be performed at much higher flow rates than in packed columns. This is particularly ...Continue Reading

References

Jan 24, 1992·Journal of Chromatography·D JosićW Reutter
Feb 7, 1992·Journal of Chromatography·P Langlotz, K H Kroner
Apr 18, 1991·Nature·F E Regnier
May 1, 1993·Biotechnology Progress·J L Manganaro, B S Goldberg
Feb 1, 1995·Analytical Chemistry·Q C WangJ M Fréchet
Feb 11, 1994·Journal of Chromatography. a·J LuksaD Josic

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·H ZouD Zhou
Jan 11, 2005·Journal of Separation Science·Fernando M LançasSilvia S de Freitas
Sep 10, 2004·Journal of Separation Science·Frantisek Svec
Nov 1, 2012·Journal of Separation Science·Fei WangXindu Geng

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