New imaging methods and tools to study vascular biology

Current Opinion in Hematology
Bàrbara Laviña, Konstantin Gaengel

Abstract

Throughout history, development of novel microscopy techniques has been of fundamental importance to advance the vascular biology field.This review offers a concise summary of the most recently developed imaging techniques and discusses how they can be applied to vascular biology. In addition, we reflect upon the most important fluorescent reporters for vascular research that are currently available. Recent advances in light sheet-based imaging techniques now offer the ability to live image the vascular system in whole organs or even in whole animals during development and in pathological conditions with a satisfactory spatial and temporal resolution. Conversely, super resolution microscopy now allows studying cellular processes at a near-molecular resolution. Major recent improvements in a number of imaging techniques now allow study of vascular biology in ways that could not be considered previously. Researchers now have well-developed tools to specifically examine the dynamic nature of vascular development during angiogenic sprouting, remodeling and regression as well as the vascular responses in disease situations in vivo. In addition, open questions in endothelial and lymphatic cell biology that require subcellular resolut...Continue Reading

References

Nov 7, 2000·Genesis : the Journal of Genetics and Development·T MotoikeT N Sato
Apr 5, 2003·Science·David J Stephens, Victoria J Allan
Jun 30, 2005·Genesis : the Journal of Genetics and Development·Stuart T FraserMargaret H Baron
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Sep 14, 2007·European Neurology·J M S Pearce
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Nov 30, 2007·Development·Xiaoqin ZhuAkiko Nishiyama
Feb 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Irina V LarinaMary E Dickinson
May 20, 2009·The Journal of Cell Biology·Mark WinderlichDietmar Vestweber
Mar 3, 2010·Nature Methods·Julia RiedlRoland Wedlich-Söldner
May 5, 2010·Cold Spring Harbor Protocols·Philipp J Keller, Ernst H K Stelzer
Oct 30, 2010·Experimental Animals·Hiroyuki IshitobiMasatsugu Ema
Dec 18, 2010·Optics Express·Florian O Fahrbach, Alexander Rohrbach
Apr 19, 2011·Genes & Development·Charles-Félix CalvoJean-Léon Thomas
Jul 19, 2011·Nature Methods·Thai V TruongScott E Fraser
Jul 23, 2011·Journal of Microscopy·A Ustione, D W Piston
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Inés Martínez-CorralSagrario Ortega
Apr 6, 2012·PloS One·Klaus BeckerHans-Ulrich Dodt
Apr 11, 2012·Genesis : the Journal of Genetics and Development·Ken MatsumotoMasatsugu Ema
Jun 5, 2012·Nature Methods·Uros KrzicLars Hufnagel
Jun 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura Fernández-MartínJaime Millán
Nov 2, 2012·PloS One·Alessia FraccaroliEloi Montanez
Nov 17, 2012·Journal of Biomedical Optics·Jing WangDan Zhu
Feb 15, 2013·Thrombosis and Haemostasis·Josef EhlingFabian Kiessling
Mar 26, 2013·Molecular Oncology·Lauri EklundKari Alitalo
Apr 12, 2013·Nature·Kwanghun ChungKarl Deisseroth
Apr 27, 2013·BMC Developmental Biology·Sonja NowotschinAnna-Katerina Hadjantonakis
Apr 30, 2013·Development, Growth & Differentiation·Takaya Abe, Toshihiko Fujimori
Jun 1, 2013·Nature Methods·Kwanghun Chung, Karl Deisseroth

❮ Previous
Next ❯

Citations

Jan 15, 2017·Trends in Endocrinology and Metabolism : TEM·Taina VuorioSeppo Ylä-Herttuala
Aug 18, 2017·JCI Insight·René HägerlingFriedemann Kiefer
Jan 4, 2017·International Journal of Molecular Sciences·Bàrbara Laviña
Mar 3, 2019·Scientific Reports·Ruslan RustMartin E Schwab

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
imaging techniques
super resolution microscopy
fluorescence microscopy
light sheet microscopy
light scattering
electron
gene trapping

Software Mentioned

STORM
CLARITY
GENSAT

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.