PMID: 7030226Nov 1, 1981Paper

New immunofluorescent blood test for gluten sensitivity

Archives of Disease in Childhood
D J UnsworthE J Holborow

Abstract

A new test for the detection of antibodies to gliadin in the sera of children who are gluten sensitive is described. This test is based on the observation that wheat protein binds selectively to connective tissue fibres in cryostat sections of mammalian tissues. Sera containing antibodies to gliadin give a reticulin pattern of staining on section pretreated with wheat gliadin if tested by indirect immunofluorescence. Antibodies to gliadin were detected in this manner in sera fom all of 32 children with coeliac disease, in 16 of whom a provisional diagnosis had been given and in 16 an established diagnosis confirmed by gluten challenge. The incidence of reticulin antibodies detected on untreated sections (in the absence of gliadin) was only 28%. Gliadin antibodies were also present in sera from 15% of 152 children with gastroenterological disorders other than coeliac disease, but most of these non-positive results for coeliac disease were in patients with transient gluten in tolerance, cows' milk-sensitive enteropathy, or Crohn's disease. This new test, although not specific for coeliac disease, promises to be of value as an indicator of gluten sensitivity, and when negative excludes a diagnosis of coeliac disease. It may also b...Continue Reading

References

Mar 1, 1979·Archives of Disease in Childhood·A M WeindlingJ Hood
May 1, 1978·Archives of Disease in Childhood·J Walker-SmithN France
Jul 26, 1975·British Medical Journal·M FairmanM Losowsky
Oct 4, 1975·British Medical Journal
Jul 1, 1975·Journal of the Science of Food and Agriculture·J A Ewart
Apr 1, 1976·Archives of Disease in Childhood·A S McNeishL J Arthur
Nov 1, 1973·Journal of Clinical Pathology·M Rizzetto, D Doniach
Aug 1, 1970·Archives of Disease in Childhood·J Walker-Smith
Feb 1, 1981·The Journal of Investigative Dermatology·D J UnsworthE J Holborow

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Citations

Jun 1, 1991·Digestive Diseases and Sciences·U VoltaF B Bianchi
Nov 1, 1984·Indian Journal of Pediatrics·V KhoshooN K Arora
Oct 14, 1983·Journal of Immunological Methods·R TronconeF Ciccimarra
Jan 1, 1996·APMIS. Supplementum·S U Friis
Jun 1, 1982·Archives of Disease in Childhood·P M Ramalho, E Monteiro
Sep 1, 1985·Annals of the Rheumatic Diseases·J T BourneJ A Wojtulewski
Sep 4, 1999·Journal of Clinical Pathology·R J LockD J Unsworth
Sep 1, 1982·The British Journal of Dermatology·J N LeonardL Fry
Sep 23, 2014·Critical Reviews in Clinical Laboratory Sciences·Vilte E BarakauskasMathew P Estey
Jul 1, 1991·Clinics in Dermatology·T P ChorzelskiE Maciejowska
Jan 1, 1984·Nutrition and Health·C M Reading
Jan 1, 1983·Annals of the New York Academy of Sciences·T P ChorzelskiV Kumar
Feb 18, 1999·The American Journal of Gastroenterology·S MoraG Chiumello
Jul 1, 1987·Acta paediatrica Scandinavica·E CacciariP Pirazzoli
Sep 1, 1984·Acta paediatrica Scandinavica·L StenhammarA Tarkowski
Dec 1, 1981·The British Journal of Dermatology·D J UnsworthL Fry
Oct 1, 1986·Scandinavian Journal of Gastroenterology·U VoltaE Pisi
Oct 1, 1986·Scandinavian Journal of Gastroenterology·E MonteiroP Magalhães Ramalho
Apr 16, 1983·Lancet·P F Bonventre
Apr 16, 1983·Lancet·D J UnsworthE J Holborow

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