New insight into the photoheterotrophic growth of the isocytrate lyase-lacking purple bacterium Rhodospirillum rubrum on acetate

Microbiology
Baptiste LeroyRuddy Wattiez

Abstract

Purple non-sulfur bacteria are well known for their metabolic versatility. One of these bacteria, Rhodospirillum rubrum S1H, has been selected by the European Space Agency to ensure the photoheterotrophic assimilation of volatile fatty acids in its regenerative life support system, MELiSSA. Here, we combined proteomic analysis with bacterial growth analysis and enzymatic activity assays in order to better understand acetate photoassimilation. In this isocitrate lyase-lacking organism, the assimilation of two-carbon compounds cannot occur through the glyoxylate shunt, and the citramalate cycle has been proposed to fill this role, while, in Rhodobacter sphaeroides, the ethylmalonyl-CoA pathway is used for acetate assimilation. Using proteomic analysis, we were able to identify and quantify more than 1700 unique proteins, representing almost one-half of the theoretical proteome of the strain. Our data reveal that a pyruvate : ferredoxin oxidoreductase (NifJ) could be used for the direct assimilation of acetyl-CoA through pyruvate, potentially representing a new redox-balancing reaction. We additionally propose that the ethylmalonyl-CoA pathway could also be involved in acetate assimilation by the examined strain, since specific en...Continue Reading

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Citations

Oct 17, 2015·Environmental Microbiology·O RevellesM A Prieto
Nov 11, 2018·Applied and Environmental Microbiology·Tetsu ShimizuMasayuki Inui
Jul 12, 2020·Applied and Environmental Microbiology·Guillaume Bayon-VicenteBaptiste Leroy
Feb 24, 2019·Archives of Microbiology·Nilüfer Afsar ErkalMeral Yücel
Jun 15, 2021·Frontiers in Microbiology·Valentine CyriaqueRuddy Wattiez

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