New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge

Scientific Reports
Lamei LiQing Huang

Abstract

We report the new insight into the dielectric barrier discharge (DBD) induced inactivation of Microcystis aeruginosa, the dominant algae which caused harmful cyanobacterial blooms in many developing countries. In contrast with the previous work, we employed flow cytometry to examine the algal cells, so that we could assess the dead and living cells with more accuracy, and distinguish an intermediate state of algal cells which were verified as apoptotic. Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect. However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells. We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment o...Continue Reading

References

Aug 4, 2011·Environmental Science & Technology·Zhigang KeZengliang Yu
Jun 26, 2012·Water Research·Pradeep RajasekharFelicity A Roddick
Apr 10, 2013·Journal of Bioscience and Bioengineering·Kazuaki NinomiyaNobuaki Shimizu

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Methods Mentioned

BETA
flow cytometry
Assay

Software Mentioned

Origin
CellQuest

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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