Dec 5, 2015

New insights in the acute toxic/genotoxic effects of CuO nanoparticles in the in vivo Drosophila model

Nanotoxicology
Mohamed AlarabyRicard Marcos

Abstract

Metal oxide nanoparticles are highly reactive from the biological point of view and, for this reason, it exists important reservations in regard human health impact. We used Drosophila as a promising in vivo model to diagnose the biological effects of copper oxide nanoparticles (CuO-NPs). Due to the potential role of ions release the effects of CuO-NPs were compared with those induced by copper sulfate, CuSO4. A wide battery of approaches has been used including toxicity, cell and body internalization, induction of reactive oxygen species (ROS) as well as changes in gene expression, related to both general stress and alterations in the intestinal barrier, and genotoxicity. The obtained results show that CuO-NPs have the ability to be distributed inside midgut cells and translocate to the general body compartment (internal hemolymph) interacting with hemocytes. Its exposure leads to reduced larval growth, decreased flies viability, delaying their emergency periods, especially at higher doses (2 and 10 mM). Moreover, deregulation of stress genes including antioxidant genes, and genes involved in wound healing were also observed. In this point it should be emphasized the novelty of using genes such as Duox, Upd3, PPO2, and Hml to ...Continue Reading

  • References61
  • Citations6

References

  • References61
  • Citations6

Citations

Mentioned in this Paper

Copper dioxide (CuO2)
In Vivo
Structure of Body Compartment
Genes
Upd3 protein, Drosophila
DUOX1 protein, human
Hemolymph
General Adaptation Syndrome
Antioxidant Effect
Antioxidants

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