New Insights Into Chromomere Organization Provided by Lampbrush Chromosome Microdissection and High-Throughput Sequencing

Frontiers in Genetics
Anna ZlotinaAlla Krasikova

Abstract

Giant lampbrush chromosomes (LBCs) typical for growing oocytes of various animal species are characterized by a specific chromomere-loop appearance and massive transcription. Chromomeres represent universal units of chromatin packaging at LBC stage. While quite good progress has been made in investigation of LBCs structure and function, chromomere organization still remains poorly understood. To extend our knowledge on chromomere organization, we applied microdissection to chicken LBCs. In particular, 31 and 5 individual chromomeres were dissected one by one along the macrochromosome 4 and one microchromosome, respectively. The data on genomic context of individual chromomeres was obtained by high-throughput sequencing of the corresponding chromomere DNA. Alignment of adjacent chromomeres to chicken genome assembly provided information on chromomeres size and genomic boarders, indicating that prominent marker chromomeres are about 4-5 Mb in size, while common chromomeres of 1.5-3.5 Mb. Analysis of genomic features showed that the majority of chromomere-loop complexes combine gene-dense and gene-poor regions, while massive loopless DAPI-positive chromomeres lack genes and are remarkably enriched with different repetitive element...Continue Reading

References

Sep 1, 1993·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·I SoloveiH Macgregor
Jan 1, 1993·The Journal of Cell Biology·J SommervilleB M Turner
Dec 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·I V SoloveiH C Macgregor
Aug 4, 1998·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·I SoloveiH Macgregor
Mar 28, 2001·Genome Génome / Conseil National De Recherches Canada·A F SaifitdinovaE R Gaginskaya
Oct 1, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Brent BeendersMichel Bellini
Sep 20, 2005·Genome Research·Belinda GiardineAnton Nekrutenko
Sep 20, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Svetlana GalkinaAlexander V Rodionov
Oct 20, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alla KrasikovaElena Gaginskaya
Oct 10, 2006·Genetica·Svetlana GalkinaElena Gaginskaya
Nov 23, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alla KrasikovaElena Gaginskaya
Aug 7, 2007·Cytogenetic and Genome Research·D K GriffinB M Skinner
Mar 25, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Christopher AustinMichel Bellini
Dec 18, 2009·Cytogenetic and Genome Research·A KrasikovaE Gaginskaya
Jul 8, 2010·Cytogenetic and Genome Research·A ZlotinaS Deryusheva
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Sep 8, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Herbert C Macgregor
Nov 13, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Anna ZlotinaSvetlana Deryusheva
Nov 13, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alla KrasikovaAnna Zlotina
Nov 15, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Garry T MorganMichel Bellini
May 29, 2014·Algorithms for Molecular Biology : AMB·Darya FilippovaCarl Kingsford
Sep 28, 2017·Chromosoma·Aleksey S KomissarovAlsu F Saifitdinova
Apr 3, 2018·Current Genomics·Tatyana Yu ZykovaIgor F Zhimulev
Dec 20, 2018·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Anna ZlotinaAlla Krasikova

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Citations

Aug 11, 2020·Molecular Cytogenetics·Tatiana KulikovaAlla Krasikova

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Datasets Mentioned

BETA
PRJEB36087

Methods Mentioned

BETA
Hi-C
phase contrast microscopy
PCR
electrophoresis
mechanical microdissection
single-cell Hi-C

Software Mentioned

RepeatMasker
sff converter
Galaxy
browser
Integrative Genomics Viewer ( IGV )
FastQC
genome
Integrative Genomics Viewer » ( IGV )
Ensembl
Bowtie2

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