New insights into circulating FABP4: Interaction with cytokeratin 1 on endothelial cell membranes

Biochimica Et Biophysica Acta
Paula SaavedraLluís Masana

Abstract

Fatty acid-binding protein 4 (FABP4) is an adipose tissue-secreted adipokine that is involved in the regulation of energetic metabolism and inflammation. Increased levels of circulating FABP4 have been detected in individuals with cardiovascular risk factors. Recent studies have demonstrated that FABP4 has a direct effect on peripheral tissues, specifically promoting vascular dysfunction; however, its mechanism of action is unknown. The objective of this work was to assess the specific interactions between exogenous FABP4 and the plasma membranes of endothelial cells. Immunofluorescence assays showed that exogenous FABP4 localized along the plasma membranes of human umbilical vein endothelial cells (HUVECs), interacting specifically with plasma membrane proteins. Anti-FABP4 immunoblotting revealed two covalent protein complexes containing FABP4 and its putative receptor; these complexes were approximately 108 kDa and 77 kDa in size. Proteomics and mass spectrometry experiments revealed that cytokeratin 1 (CK1) was the FABP4-binding protein. An anti-CK1 immunoblot confirmed the presence of CK1. FABP4-CK1 complexes were also detected in HAECs, HCASMCs, HepG2 cells and THP-1 cells. Pharmacological FABP4 inhibition by BMS309403 res...Continue Reading

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Citations

Feb 7, 2019·Journal of Atherosclerosis and Thrombosis·Masato Furuhashi
Feb 2, 2019·Journal of Lipid Research·Kacey J PrenticeGökhan S Hotamisligil
Apr 14, 2017·Pflügers Archiv : European journal of physiology·Yuta OkamuraHideyuki Yamawaki
Nov 11, 2019·Diagnostics·Ekaterina M StakhnevaYuliya I Ragino
Dec 7, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·N Martínez-MicaeloL Masana
Mar 16, 2017·Open Access Macedonian Journal of Medical Sciences·Alexandr Borisovich MarchenkoAnar Akyilbekovna Turmukhambetova
Jul 23, 2021·Progress in Lipid Research·Rahul MallickAsim K Duttaroy

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